摘要
在单因素试验的基础上,利用响应曲面法(response surface methodology,RSM),通过中心组合设计,对蕨菜多糖提取工艺参数进行优化分析研究。选择料水比、提取温度和提取时间作为优化因子,研究了各因子对蕨菜多糖得率的影响。通过分析得到优化多糖的提取条件:水料比18.8:1(V/W),提取温度为62.5℃;时间5.9h。在此条件下蕨菜多糖提取得理论值达到2.06%,实际最大多糖得率为2.02% ± 0.16%。同时,用 FARP 法测定了多糖的抗氧化活性。
The optimum conditions for the extraction of the water-soluble polysaccharides from Pteridium aquilinum were determined using response surface methodology （RSM）. A central-composite experimental design （CCD） was used to investigate the interaction effects of three independent variables, namely liquid-solid ratio, extraction temperature and extraction time. Also, the antioxidant activity of the crude polysaccharides obtained from Pteridium aquilinum was investigated in this study. The optimum conditions are as follows： liquid-solid ratio 18.8：1 （V/W）, extraction temperature 62.5 ℃ and extraction time 5.9 h. The crude polysaccharide shows excellent antioxidant activity according to FARP antioxidative assay.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第7期122-126,共5页
Food Science
基金
国家863项目(2001AA212291)
关键词
响应曲面法
多糖
抗氧化性
蕨菜
response surface methodology
polysaccharides
antioxidatnt activity
Pteridium aquilinum
