摘要
本实验探索了一种反胶束法提取蛋白质的后萃方法,即先回收异辛烷,然后用少量KCl的缓冲溶液溶解剩余的固形物,最后用丙酮沉淀得小麦胚芽蛋白。研究了KCl浓度、缓冲液pH值、缓冲液加入量对小麦胚芽蛋白后萃率的影响,并在单因素基础上,通过响应面分析法确定后萃最佳工艺条件为:KCl浓度0.61mol/L、缓冲液pH9.47、缓冲液加入量1.0ml,在此工艺条件下,小麦胚芽蛋白后萃率达到80.07%。
In this study, backward extraction procedure was explored for wheat germ protein. After recovery of isooctane and dissolution of residual solid with a small amount of KCl buffer solution, the wheat germ protein was precipitated by acetone, while AOT remained in the acetone phase. The effects of KCl concentration, pH value and the amount of buffer solution on the backward extraction rate were investigated. On the basis of single-factor tests, the optimum backward extraction conditions were achieved by response surface methodology, namely KCl concentration 0.61 mol/L, pH value 9.47 and the amount of buffer solution 1.0 ml. Under these technical conditions, the backward extraction rate of protein reaches 80.07%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第7期208-212,共5页
Food Science
基金
“十五”国家重点科技攻关项目(2001BA501A04)
高等学校博士学科点专项科研基金项目(20070295034)
关键词
反胶束
小麦胚芽蛋白
后萃
响应面分析
reverse micelles
wheat germ protein
backward extraction
response surface methodology
