摘要
本实验对ε-聚赖氨酸产生菌-白色链霉菌的原生质体制备、再生条件进行了研究,建立了白色链霉菌原生质体诱变体系。在此基础上,通过对原生质体进行脉冲光诱变,氦氖激光诱变,筛选得到一株高产菌株;通过双层平板实验证明比出发菌株抑菌圈的H/C大0.28～0.45cm;通过发酵证明比出发菌株产ε-聚赖氨酸的能力提高40%以上。
To screen high ε-polylysine-producing mutants of Streptomyces albulus, the methods of protoplast preparation, regeneration and mutagenesis were established. Thereafter, the protoplasts of the original strain were treated by pulsed-ligh and He-Ne laser. The double layer culture medium test proved that the H/C value of mutation is 0.28～0.45 cm, bigger than that of the original strain. The fermentation result showed its ε-polylysine productivity is higher 40% than that of the original strain.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第7期277-280,共4页
Food Science
基金
辽宁省教育厅科技攻关项目(2004D166)
