摘要
目的建立高效液相色谱法测定注射用甲磺酸左氧氟沙星的含量。方法 采用ODS2色谱柱（5μm，4．6mm×200mm），乙腈-0．05mol·L^-1磷酸二氢钾溶液（pH2．2）-0．05mol·L^-1四丁基澳化铵溶液（116：861：23）为流动相，流速1mL·min^-1，检测波长293nm，外标法计算含量。结果甲磺酸左氧氟沙星在20μg·mL^-1～200μg·mL^-1的浓度范围内，线性关系良好，回归方程Y=55667．78X＋10．32886，r=0．99998；系统精密度RSD为0．1％（n=6）。结论本方法操作简便、快捷，方法的精密度、准确度均能很好地满足质量控制的要求，适合于注射用甲磺酸左氧氟沙星的含量测定。
OBJECTIVE To Establish a HPLC method for the determination of the content of levofloxacin mesylate for injection. METHODS The HPLC system consisted of Elite Hypersil ODS2 column （5μm, 4.6mm × 200mm）, acetonitrile-0.05mol · L^-1 potasssium dihydrogen phosphate solution （ pH2.2 ） - 0.05mol · L^-1 tebrabutyl ammonium bromide （116：861：23） as mobile phase with the flow rate of 1mL·min^-1, and UV detector wavelength was 293nm, calculated with the external method. RESULTS Under the condition, there was a good linear relationship in the concentration range of 20μg·mL^-1～ 200μg·mL^-1（r = 0. 99998）, the system precision （RSD = 0.1% n = 6） was good. CONCLUSIONS This method is simple, rapid, precise, and accurate, providing an effective way in quality control, and is suitable for the determination of the content of levofloxacin mesylate.
出处
《海峡药学》
2008年第7期52-53,共2页
Strait Pharmaceutical Journal
