摘要
目的:探讨拓扑替康(TPT)对人髓系白血病细胞株HL-60细胞凋亡的影响。方法:取倍增期的HL-60细胞分组培养,实验组培养液中加入0.15μmol/L的TPT,空白对照组继续常规培养。分组培养4h、12h后分别采用流式细胞仪AnnexinV-FITC染色及TUNEL法检测细胞凋亡情况。结果:分组培养4h后,实验组细胞凋亡率为(28.57±3.16)%,高于空白对照组的(3.12±0.38)%,差异具有统计学意义(t=13.85,P=0.0002)。分组培养12h后,实验组TUNEL阳性细胞数达38.33%,高于空白对照组的2.67%(χ2=117.08,P<0.05)。结论:拓扑替康具有诱导HL-60细胞凋亡的作用。
Aim ： To investigate the inhibitory effect of TPT on HL-60 cells apoptosis. Methods： HL-60 cells were allocated into 2 groups, cells in the experimental group were incubated with 0.15μmol/L TPT, and cells in the control group were incubated regularly. The apoptosis of HL-60 cells were detected by FCM Annexin V-FITC when incubated for 4 h and TUNEL method when incubated for 12 h. Results：After 4 h of the treatment, the apoptosis rate of experimental group was （ 28.57 ± 3.16 ） %, higher than （ 3.12 ± 0.38 ） % of the control group （ t = 13.85, P = 0.000 2）. When treated with 0.15 p.mol/L TPT for 12 h, the proportion of TUNEL positive cells was 38.33% , significantly higher than 2.67% of the control group （X2 =117.08, P 〈 0.05 ）. Conolusion： TPT could induce apoptosis of HL-60 cells.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2008年第4期704-706,共3页
Journal of Zhengzhou University(Medical Sciences)
