摘要
为探讨p72、AIB1、erbB-2与雌激素受体(ERα)活性关系及意义,采用定量RT-PCR技术,检测不同分化程度的子宫内膜癌组织中ERa及其协同因子p72、AIB1、erbB-2的mRNA表达水平,并进行荧光素酶检测分析ERα活性与p72、AIB1、erbB-2表达的关系。结果显示,p72、AIB1和erbB-2的共同表达,促使雌激素受体的活性呈叠加增长。推测p72、AIB1与erbB-2对雌激素受体激活有相互促进作用。
To determine how estrogens are involved in the growth of endometrial cancers we investigated the status of ERα and its cofactors p72 ,AIB1 and erbB--2 in endometrial cancers with varying degrees of differentiation by real--time RT--PCR,and their in vivo cellular effects on the transactivation function of ERa were examined by a transient expression assay. Results showed that the mRNA levels of p72,AIB1 and ERα decreased and those of erbB--2 increased with the loss of histological differentiation. Transient expression of p72 ,AIB1 and erbB--2 in human embryonic kidney 293T cells led to a synergistic promotion of the transactivation function of ERα in the presence of 17α--estradiol or 4--hydroxytamoxifen,an ERα AF--1 agonist/AF--2 antagonist,as a ligand. In conclusion, estrogen action through ERα AF--1 might be exerted by the increased expression of the coactivators,p72 and AIB1 ,together with cross talk between erbB--2 and p72 to accelerate the transactivation of ERa AF--1 in endometrial cancer.
出处
《医学与哲学（B）》
2008年第7期43-45,共3页
Medicine & Philosophy(B)
