摘要
2-苯乙醇对酵母的抑制作用是生物法合成2-苯乙醇的瓶颈之一,研究酵母对2-苯乙醇耐受性有可能使2-苯乙醇产量大幅提高.通过用紫外诱变等方法对酿酒酵母Saccharomyces cerevisiae CWY132进行诱变育种,获得了10株2-苯乙醇耐受性菌株.对耐受性突变酵母菌株的2-苯乙醇耐受性与产量之间的关系进行了考察,其中CWY132-10菌株的耐受性和产量都较高,耐受性较原始菌株提高了50%,产量提高了9.1%,摩尔转换率达到97.4%.
The inhibition of 2-phenylethanol to yeast is one of the most difficult problems in the production of 2-phenylethanol by Saccharomyces cerevisiae. In this study, Saccharomyces cerevisiae CWY132 was treated by Ultraviolet radiation, and ten resistance mutants were screened out by the medium containing high concentration of 2-phenylethanol. Studies on the relations between the resistance and the yield of 2-phenylethanol showed that CWY132-10 strain presented the highest tolerance to 2-phenylethanol and highest yield of 2-phenylethanol, its resistance increased by 50% and the yield of 2-phenylethanol increased by 9. 1% compared with the original strain, the molar conversion rate of L-phenylalanine to 2-phenylethanol is 97. 4%.
出处
《浙江工业大学学报》
CAS
2008年第4期427-430,共4页
Journal of Zhejiang University of Technology
关键词
酿酒酵母
2-苯乙醇
耐受性
诱变选育
Saccharomyces cerevisiae
2-phenylethanol
tolerance
induced mutation breeding
