摘要
Ｎ＋注入陆地棉和海岛棉的成熟花粉用于自交和远缘杂交，结果表明：离子注入花粉，在Ｍ１代就可表出现较高频率的有益突变，在Ｍ１相对成苗率和花粉育性为５０％时，花粉辐照Ｍ２突变率是种子辐照的１．６倍，用Ｎ＋离子注入陆地棉和海岛棉花粉进行正反远缘杂交，Ｆ２Ｍ２农艺性状表现出偏母遗传且后代遗传稳定加快，并观察到父本某些性状的转移。离子注入花粉适宜的剂量范围为２×１０１５—１０×１０１５Ｎ＋／ｃｍ２。
The ripe pollen, treated by Nitrogen ion beam, of island cotton and upland cotton were applied to selfing and distant hybridizing . The result has indicated that the N + implantation of the pollens can result in higher beneficial mutation frequency in M 1 generation .While the relative survival seedling rate and pollen fertility are 50%, the M 2 mutation frequency of pollen ion irradiation is 1.6 times the one of seed ion irradiation . The island and upland cotton pollens, implanted by N +, were utilized to perform forward and back distant crosses. The F 2M 2 agrionomic characters have matroclinal inheritance, and the genetical stability of the descendants is accelerated; some paternal charaters'transfer are observed. The suitable dosage range of nitrogen ion implantation of pollens is between 2×10 15 N +/cm 2 and 10×10 15 N +/cm 2.
出处
《激光生物学报》
CAS
CSCD
1997年第3期1160-1164,1159,共6页
Acta Laser Biology Sinica
