期刊文献+

鸡肌球蛋白轻链激酶表达载体的构建 被引量:4

Construction of Chicken Myosin Light Chain Kinase Expression Vector
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摘要 鸡肌球蛋白轻链激酶(MLCK)在调节平滑肌细胞收缩中具有重要作用.用PCR产生构建所需的限制性内切酶SalⅠ位点,将鸡MLCKcDNA插入质粒pBKrsv中,构建成pBKrsv-MLCK,并通过酶切图谱、SalⅠ位点序列分析得到证实.重组质粒在大肠杆菌中获得表达. A MLCK cDNA fragment containing SalⅠ site was generated by Polymerase Chain Reaction (PCR).The pBKrsv MLCK was constructed by inserting MLCK cDNA into pBKrsv polylinker and identified by restriction mapping and sequence analysis of SalⅠ site.These results provides fundation for further investigation of MLCK expression.
作者 汪渊 桂淑玉
出处 《生物化学杂志》 CSCD 1997年第5期520-524,共5页
关键词 肌球蛋白 轻链激酶 表达载体 Myosin light chain kinase (MLCK),pBKrsv,Restriction mapping,cDNA,Sequencing,Chicken
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参考文献1

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同被引文献24

  • 1Bin Ren~1 Hua-Qing Zhu~2 Zhao-Feng Luo~1 Qing Zhou~2 Yuan Wang~2 Yu-Zhen Wang~1 1 Department of Biochemistry and Molecular Biology,University of Science and Technology of China,Hefei 230027,Anhui Province,China2 Laboratory of Molecular Biology,Anhui Medical University,Hefei 230032,Anhui Province,China.Preliminary research on myosin light chain kinase in rabbit liver[J].World Journal of Gastroenterology,2001,7(6):868-871. 被引量:6
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