摘要
利用基因工程重组技术获得了绿色荧光蛋白（ｇｆｐ）基因与ＨＣＶ核心蛋白（ｃｏｒｅ）基因的嵌合体，并在大肠杆菌中高效表达了４８ｋＤａ的融合蛋白，经ＤｏｔＥＬＩＳＡ和Ｗｅｓｔｅｒｎｂｌｏｔ免疫活性分析证实，融合蛋白仍具有ｃｏｒｅ抗原的三个免疫活性部位，同时用荧光显微镜观察并用荧光光度计测定了大肠杆菌表达的融合蛋白的荧光光谱，结果证实，我们在大肠杆菌中表达的ＧＦＰｃｏｒｅ融合蛋白既能发射易于检测的绿色荧光，又具有ＨＣＶ核心蛋白的抗原活性，实现了用绿色荧光蛋白等分子标记抗原，为免疫诊断新方法的建立，打下了理论基础。
A chimeric gene of the green fluorescent protein and hepatitis C virus core antigene was constructed and expressed in E.coli cells.The expressed fusion protein was examined by Dot ELISA and Western blot and the three antigenic determinants were detected.The GFP core fusion protein has not only the striking green fluorescence under natural light but also the HCV antigenic activity.A new methord of immunological diagnosis is highly expected on the light of the fusion protein which can be seen as HCV antigen tagged by equal green fluorescent protein.
出处
《生物工程学报》
CAS
CSCD
北大核心
1997年第4期368-374,共7页
Chinese Journal of Biotechnology
基金
自然科学基金
关键词
丙型肝炎病毒
核心蛋白
绿色荧光蛋白
融合蛋白
HCV core gene,green fluorescent protein gene,fusion protein ,fluorescent spectrum
