摘要
植物转录因子在植物的生长发育及其对外界环境的反应中起着重要的作用。bHLH转录因子是植物转录因子最大的家族之一。本研究以水稻日本晴为材料,在苗期进行PEG模拟干旱胁迫和ABA(abscisicacid)处理,采用荧光定量PCR方法,比较分析干旱胁迫差异表达的22个候选bHLH家族基因在PEG和ABA处理下的不同时间梯度的表达谱。结果表明大部分候选基因的PEG胁迫表达谱与干旱胁迫表达谱一致,验证了芯片结果的可靠性;而在ABA处理下的大部分bHLH基因表现为抑制表达,部分基因的表达谱与干旱或PEG胁迫表达谱明显不同,推测这些bHLH基因参与PEG和ABA胁迫应答的具有相同或不同的分子路径或模式。研究结果对进一步克隆这些基因及分析其在植物生长发育过程中的分子调控模式打下了基础。
Transtription factors have a central regulating role in plant development, differentiation and response to environmental stresses. The bHLH family is one of the biggest transcription factor families in plant. To comparative analyze the expression patterns of candidate bHLH genes, which were differentially expressed under drought stress, under PEG-simulated drought stress and ABA treatment, quantitative real time PCR was used to profile their expression changes of the rice seedling under the treatment. Results showed that the alteration of genes ex- pression under PEG stress was consistent with that of drought stress detected by microarray analysis; but in most cases the candidate bHLH genes were down-regulated under ABA treatment, which was obviously different from that under drought or PEG stress, it was speculated that these bHLH genes might be involved in the responsiveness to PEG and ABA stress by the same or different molecular pathway or mode. Our results will provide a basis of these bHLH genes cloning and dissection of their molecular mechanism in plant growth and development.
出处
《分子植物育种》
CAS
CSCD
2008年第3期425-431,共7页
Molecular Plant Breeding
基金
国家973项目(2003CB114308)资助
关键词
水稻
转录因子
ABA
表达谱
Rice, Transtription factor, ABA, Expression pattern
