摘要
鱼白细胞介素-2是近年来新发现的一类细胞因子。根据Bird等发表的河豚IL-2基因序列设计一对特异性引物，通过RT—PCR方法扩增和克隆了红鳍东方纯（Fugum6却es）白介素-2的核苷酸序列。该cDNA序列由490nt组成，编码由149个氨基酸组成的前体蛋白。序列分析表明，红鳍东方纯IL-2核苷酸序列和氨基酸序列与目前已知的其它物种IL-2的核苷酸及氨基酸的同源性分别位于34％-44％和24％～43％之间，N端存在长22个氨基酸的信号肽，含有形成2个链内二硫键的4个半胱氨酸。遗传进化分析表明，红鳍东方纯IL-2在遗传进化上具有其独立性。
Fish interleukin-2 was a kind of important cytokine discovered recently. One pair of specific primers were designed according to IL-2 gene sequence published by Bird et al, IL-2 gene of Fugu rubripes was cloned by RT-PCR method with mRNA extracted from blood lymphocytes activated by PHA. Sequencing results showed that the gene was 450 bp in length, encoding a preprotein of 149aa residues, formation has the two cysteines important in disulphide-bond formation. It shows low amino acid identities （24%-34%） with other known IL-2 sequences. Phylogenetic analysis showed far relationships between Fugu rubripes and other species.
出处
《上海水产大学学报》
CSCD
北大核心
2008年第4期497-501,共5页
Journal of Shanghai Fisheries University
基金
浙江万里学院人才引进启动基金(1741000171)
浙江省教育厅科研项目(20061024)
科技部国家星火计划项目(2006EA701002)
