摘要
目的从对溃疡性结肠炎大鼠结肠成纤维细胞增殖影响的角度,探讨艾灸防治溃疡性结肠炎肠纤维化的作用机制。方法采用免疫法加局部刺激制备溃疡性结肠炎肠纤维化大鼠模型,随机将大鼠分为正常组、模型组、隔药灸组、温和灸组和西药组。隔药灸组和温和灸组选取天枢、气海穴分别进行隔药灸和温和灸治疗,西药组柳氮磺胺吡啶溶液灌胃治疗,治疗结束后处死大鼠,剖取结肠组织,分离并培养结肠成纤维细胞,流式细胞术观察各组大鼠结肠成纤维细胞增殖情况。结果模型大鼠结肠成纤维细胞增殖率增高(P<0.01),处于G0-G1期细胞减少(P<0.01),G2-M期和S期细胞增多(P<0.01),隔药灸、温和灸疗法能显著改善大鼠结肠成纤维细胞的增殖率(P<0.01),使处于G0-G1期细胞、G2-M期和S期细胞趋向正常。结论UC大鼠CFB增殖加快,隔药灸和温和灸天枢、气海穴治疗能显著抑制大鼠CFB的过度增殖。
Objective To investigate the mechanisms of moxibustion in the treatment of the colonic fibrosis in ulcerative colitis （UC） by observing the colonic fibroblast （CFB） proliferation in ulcerative colitis fibrosis rats. Methods A rat model of ulcerative colitis fibrosis was established by immunological methods using human colonic mucosa as antigen adding local stimulation. The rats were randomly divided into normal group, model group, herb-partition moxibustion group, mild-warm moxibustion group and western medicine group （SASP group）. Herb-partitioned rnoxibustion group and mild-warm moxibustion group treated by herb-partitioned moxibustion and mild-warm moxibustion respectively on Qihai （ CV 6 ） and Tianshu （ ST 25, bilateral） points. SASP group fed with salicylazosulfapyridine. Colonic fibroblasts from all the rats were isolated and cultured and the proliferation of colonic fibroblasts was determined by flow cytometry. Results Colonic fibroblasts from ulcerative colitis fibrosis rats＇ conlon proliferated faster than those of normal ones. The Colonic fibroblasts number in model group in G0-G1 period decreased（ P 〈 0.01 ） , in G2- M period and S period increased （P 〈 0.01 ）. Treatment of herbs-partition moxibution and mild-warm rnoxibution can inhibit its proliferative index. Conclusion Moxibution on Qihai （ CV 6） and Tianshu （ ST 25, bilateral） points is effective in preventing the over proliferated of colonic fibrosis in ulcerative colitis fibrosis rats.
出处
《上海针灸杂志》
2008年第7期42-45,共4页
Shanghai Journal of Acupuncture and Moxibustion
基金
国家自然科学基金项目资助(30400609)
上海市青年科技启明星项目资助(06QA14049)
上海市重点学科建设项目资助(T0302)
关键词
针灸疗法
结肠炎
溃疡性
成纤维细胞
增殖
大鼠
Acupuncture-Moxibustion Therapy
Colitis, Ulcerative
Fibroblasts
Proliferation
Rats
