摘要
目的:建立宝宝乐颗粒中芍药苷的高效液相色谱(HPLC)测定方法。方法:采用Diamonsil C18(4.6×250mm,5um)色谱柱,以乙腈-水-磷酸(20:80:0.05)为流动相,检测波长:230nm,流速:1.0mL/min,柱温:35.0℃。结果:芍药苷在0.1540ug^1.2320ug范围内与峰面积线性关系良好,相关系数为r=0.9999,平均回收率为99.82%(RSD=0.301%,n=5)。结论:HPLC法操作快速、简便、结果准确,可作为宝宝乐颗粒中芍药苷的定量分析方法。
To establish a HPLC method for content determination of the paeoniflorin in baobaole granule, the paper uses the following methods： a Diamonsil C18 column serves as the chromatographic column（4.6×250mm, 5um）, the mobile phase consists of acetonitrile-water-phosphoric acid （20：80：0.05）, the UV detection wavelength is 230nm ,the flow rate is 1.0mL / min and the column temperature is 35.0℃. The results show that： there is a good linear relationship between the samplesize of the paeoniflofin and peak area score when the samplesize is within the range of 0. 1540ug - 1. 2320ug, the relative coefficient（r） is 0. 9999 and the average recovery is 99.82 % （ RSD = 0.301% ,n =5）. Thus we get the conclusion： The HPLC method is provided to be quick, convenient and accurate, and may be used as quantitative analytical method of paeoniflofin in baobaole granule.
出处
《贵州教育学院学报》
2008年第6期17-19,共3页
Journal of Guizhou Educational College(Social Science Edition)
