摘要
分别以丰花月季叶片、叶柄和茎段为外植体,进行了愈伤组织的诱导及植株再生的研究。结果表明,丰花月季诱导愈伤组织最佳激素浓度配比是1/2MS+2,4D 5 mg.L-1+BA 1 mg.L-1;所得到的愈伤组织在MS+6-BA1 mg.L-1+2,4D 2.5 mg.L-1+GA3 1 mg.L-1的培养基上继代保存两个月后于MS+NAA 0.02 mg.L-1+TDZ 1.8 mg.L-1+KT1 mg.L-1上分化出不定芽;不定芽在1/2MS+IBA 0.5 mg.L-1上的生根率为91.25%。
Plant regeneration system was established from the callus tissue which was induced from leaf, leaf-stalk and stem segments of Rose floribunda. The result showed that the callus was obtained when cultured on 1/2MS medium with 2, 4D 5 mg·L^-1, 6-BA 1 mg·L^-1. After the callus was maintained on MS medium with 6-BA 1 mg·L^-1, 2, 4D 2.5 mg·L^-1 and GA3 1 mg·L^-1 for two months, adventition buds could be induced from the callus on MS medium with NAA 0.01 mg·L^-1, TDZ 1.8 mg·L^-1 and KT 1 mg·L^-1, and 1/2MS medium with IBA 0.05 mg·L^-1 was the optimum shoot rooting medium with 91.25% rooting.
出处
《东北农业大学学报》
CAS
CSCD
2008年第6期30-32,共3页
Journal of Northeast Agricultural University
