期刊文献+

三氧化二砷与HMBA对白血病K562细胞增殖抑制作用及其分子机制的研究 被引量:5

Inhibitory effect of As_2O_3 and HMBA on the multiplication of K562 cell and its molecular mechanism
下载PDF
导出
摘要 目的:探讨诱导分化剂对K562细胞增殖抑制的作用与分子机制。方法:通过MTT试验和细胞形态学观察K562细胞增殖的改变和分化情况。通过流式细胞仪实验检测细胞周期改变,RT-PCR检测EVI1、TGF-β1和WT1基因表达的变化。结果:K562细胞经六亚甲基二乙酰胺(HMBA)和(或)三氧化二砷(As2O3)作用后增殖明显受到抑制,并向不同方向分化,细胞被阻滞在G1期。K562细胞的增殖抑制作用与EVI1和WT1基因表达下调,TGF-β1基因表达上调一致。EVI1/β-actin从0.9253渐降至0.1379,WT1/β-actin从0.9953降至0.1978。TGF-β1/β-actin从0.3315增至1.2452。结论:HMBA、As2O3及其联合抑制K562细胞增殖作用与EVI1和WT1基因表达下调,TGF-β1基因表达上调有关。 OBJECTIVE: To analyze the inhibitory effect and the molecular mechanism of the induction differentiation on the multiplication of K562 cell. METHODS: The multiplication and differentiation of K562 cell were observed through the MTT experiment and the cytomorphology. The flow cytometer was used to examine the cell cycle changes, and the RT-PCR was used to test EVIl, TGF-β1and WT1 gene expression changes. RUSULTS: The muhiplication of was inhibitated K562 cell was inhibitated obviously was inhibitated by HMBA or/and As2O3. The cells were hindered in the G1 stage, which was consistent to EVIl and the WT1 gene expression declines, and TGF-β1 gene expression upward. EVI1/β-actin fell deereased from 0. 925 3 to 0. 137 9, and WT1/β-actin fell from 0. 995 3 to 0. 197 8. TGF-β1/β-actin increased from 0. 331 5 to 1. 245 2. CONCLU- SION: HMBA or/and As2O3 suppresse the K562 cell multiplica tion function which is related ro EVIl and the WT1 gene expression declines and TGF-β1 gene expression upward.
出处 《中华肿瘤防治杂志》 CAS 2008年第14期1049-1053,共5页 Chinese Journal of Cancer Prevention and Treatment
基金 国家自然科学基金(30771103) 科技部中瑞政府间合作项目(AM15B 1) 山东省优秀中青年科学家基金项目(03BS033) 山东省自然科学基金重点项目(Z2004C08) 山东省自然科学基金(Y2005C39) 山东省卫生高层次人才1020工程专项经费资助
关键词 白血病 K562细胞 乙酰胺类 砷剂 leukemia K562 cells acetamides arsenicals
  • 相关文献

参考文献8

  • 1Andersson L C, Jokinen M, Gahmberg C G. Induction of erythroiddifferentiation in the human leukemiacellline K562 [J]. Nature,2005,278(3) :364-370.
  • 2Alitalo R. Induceddifferentiation of K562 leukemiacells: Amodel forstudies of geneexpressioninear lymegakaryoblasts[J]. Leuk Res,1990,14(6) :501-508.
  • 3Yuasa H, Oike Y, Iwama A. Nishikata Ⅰ oncogenic transcription factor Evil regulates hematopoietic stem cell proliferation through GATA 2 expression[J]. EMBO J,2005.24(11) :1976-1987.
  • 4Kinuko M. Molecular mechanisms of leukemogenesis by AML1/ EVI-1 1Department of Hematology [J]. Dokkyo University School Medicine, Tochigi 321-0293, Japan
  • 5Kahata K, Asaka M, Miyazono K. TGF beta signaling and carcinogenesis[J].Nippon Rinsho,2005,63(Supp14):549-554.
  • 6Hirai H, Izutsu K, Kurokawa M,et al. Oncogenic mechanisms of Evi-1 protein[J]. Cancer Chemother Pharmacol, 2001,48 Suppl: S35-40.
  • 7Alliston T, Ko T C, Cao Y, et al. Repression of bone morphogenetic protein and activin-inducible transcription by Evi-1[J]. J Biol Chem, 2005, 280(25): 24227-24237.
  • 8Mayo M W, Wang C Y, Drouin S S, et al. WT1 modulates ap optosis by transcriptionally upregulating the bcl-2 proto-oncogene[J]. EMBOJ, 1999, 18(14): 3990-4003.

同被引文献79

引证文献5

二级引证文献16

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部