摘要
目的研究我国延边地区淋巴瘤与鼻咽癌中EB病毒类型的分布特点。方法采用聚合酶链反应(PCR),巢式PCR和限制性酶切技术分析检测延边地区32例淋巴瘤与31例鼻咽癌中EB病毒的类型分布。结果在延边地区,22%的淋巴瘤和81%的鼻咽癌表现为EBNA-1阳性。EBNA-1阳性的7例淋巴瘤均表现为EB病毒-1型和"F"型,其中4例(57%)为"C"变异型;而在EBNA-1阳性的25例鼻咽癌中,22例(88%)为EB病毒-1型,3例为EB病毒-2型。除1例"f"变异型以外,均为"F"型,"C"变异型占鼻咽癌的63%(10/16)。结论EB病毒-1型为延边地区的主要EB病毒株,Bam HI"f"变异型罕见。
Objective To investigate the distribution of different Epstein-Barr virus （EBV） types in Yanbian of China. Methods Polymerase chain reaction（PCR）, nested-PCR and restriction enzyme cutting technology were used for EBV screening and typing. Results In the cases from Yanbian of China,22%（7/32） of MLs and 81%（25/31 ） nasopharyngeal carcinomas（NPCs） were positive for EBNA-I. EBV was classified as type 1 and ＂F＂ in all 7 EBNA-1 positive MLs,in which 4 cases were ＂C＂ variant. However,the virus in NPCs were classified as type 1 in 22（88% ）,and type 2 in 3 （12%）. All cases were ＂F＂ except one ＂f＂ variant case. And ＂C＂ variant was 63%（ 10/16）in NPCs. Conclusion EBV type 1 is ubiquitous strain in Yanbian of China. And BamHI ＂f＂ variant is rare.
出处
《中国现代医生》
2008年第21期44-46,共3页
China Modern Doctor
基金
延边大学归国留学博士启动基金资助
关键词
淋巴瘤
鼻咽肿瘤
疱疹病毒4型
人
病毒基质蛋白质类
Malignant lymphoma
Nasopharyngeal neoplasms
Herpesvirus, human
Viral matrix proteins
