期刊文献+

感染性脑损伤中的细胞凋亡及凋亡诱导因子的表达及意义 被引量:1

Cell Apoptosis and the Expression of Apoptosis-inducing Factor in Rats Brain Infectious Injury
下载PDF
导出
摘要 目的:探讨凋亡在感染性脑损伤的发生发展以及caspase非依赖性途径在感染性脑损伤机制中的作用。方法:SD大鼠160只,随机分为NS组和LPS组各80只,LPS组颈内动脉注射LPS制作感染性脑损伤模型,NS组颈内动脉注射NS,2组均观察注射后6、12、24、48及72h5个时间点,检测各组不同时间点脑组织的EB含量,免疫组化检测脑组织NSE、GFAP蛋白及凋亡诱导因子(AIF)的表达,并应用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)技术检测细胞凋亡数。结果:LPS组NSE、GFAP、AIF表达及细胞凋亡数均为6h开始增加,12h表达进一步增强,24h达高峰,48-72h时渐减少,但均明显高于NS组。结论:细胞凋亡参与感染性脑损伤细胞发展过程,Caspase非依赖性途径通过AIF的表达发挥重要作用。 Objective: To explore the effect of apoptosis-inducing factor (AIF) and the caspase indeperident pathway in infectious brain injury. Methods: Models of acute infectious brain injury of rats were prepared by injecting LPS via the left internal carotid artery. One hundred and sixty rats were randomly divided into NS and LPS group, At 6 h,12 h,24 h,48 h and 72 h after acute infectious brain injury, ethidium bromide(EB) content was measured by forrnamide-method, the expression of NSE protein, GFAP protein and AIF were studied by immunohistochemistry, and apoptosis was examined by TUNEL technique. Results:The expression of NSE, GFAP, AIF and the number of apoptosis in LPS group was increased at 6 h and reached the peak at 24 h, and then decreased gradually at 48- 72 h, but still significantly higher than in NS group. Conclusion:Apoptosis plays a role in the formation and development of infectious brain injury of rats. Caspase independent pathway was involved in this process through AIF.
出处 《神经损伤与功能重建》 2008年第4期231-234,共4页 Neural Injury and Functional Reconstruction
关键词 感染性脑损伤 细胞凋亡 凋亡诱导因子 LPS infectious brain injury cell apoptosis AIF LPS
  • 相关文献

参考文献17

  • 1丛志强,李文馨,等.化脓性脑膜炎的治疗进展[J].临床神经病学杂志,2001,14(2):123-125. 被引量:6
  • 2戴永建,戢翰升.醒脑静注射液对大鼠脑损伤后细胞凋亡及相关蛋白表达的影响[J].中国临床神经外科杂志,2006,11(9):551-553. 被引量:29
  • 3TWEEDIE D, MILMAN A, HOLLOWAY HW, et al. Apoptotic and Behavioral Sequelae of Mild Brain Trauma in Mice[J]. J Neurosci Res(S0360-4012) ,2007,85(4) : 805-815.
  • 4ZHU C, WANG X, HUANG Z,et al. Apoptosis-inducing Factor is a Maior Contributor to Neuronal Loss Induced by Neonatal Cerebral Hypoxia-ischemia [J]. Cell Death Differ (S1350 - 9047), 2007,14 (4) : 775 - 784.
  • 5ZHU C, XU F, WANG X,et al. Different Apoptotic Mechanisms are Activated in Male and Female Brains after Neonatal Hypoxia-ischemia [J]. J Neurochem (S0022 - 3042 ), 2006,96 (4) : 1016 - 1027.
  • 6BIFRARE YD, KUMMER J, JOSS P, et al. Brain- Derived Neurotrophic Factor Protects Against Multiple Forms of Brain Injury in Bacterial Meningitis[J]. J Infect Dis(S0022 - 1899), 2005,191 (1) : 40- 45.
  • 7WEI G, WANG D, LU H,et al. Intranasal Administration of a PARG Inhibitor Profoundly Decreases Ischemic Brain Injury[J]. Front Biosci (S1093- 9946), 2007,12 : 4986- 4996.
  • 8SMITH FM, RAGHUPATHI R, MACKKINNON MA, et al. TUNEL-positive Staining of Surface Contusions after Fatal Head Injury in Man[J]. Acta Neuropathol (Berl) (S0001 - 6322), 2000,100 (5): 537- 545.
  • 9于翠娟,王成济,杨安钢.AIF及AIF依赖的细胞凋亡[J].国外医学(分子生物学分册),2003,25(6):351-354. 被引量:10
  • 10ZHANG Z, ZHANG Z, ARTELT M, et al. Dexamethasone Attenuates Early Expression of Three Molecules Associated with Microglia/Macrophages Activation Following Rat Traumatic Brain Injury [J]. Acta Neuropathol(S0001- 6322) ,2007,113(6) :675-682.

二级参考文献57

  • 1王宁生,梁美蓉,刘启德,叶少梅.冰片“佐使则有功”之实验研究[J].中医杂志,1994,35(1):46-47. 被引量:84
  • 2[1]Susin SA et al. J Exp Med, 1996; 184(4): 1331
  • 3[2]Susin SA et al. Nature, 1999; 397(6718): 441
  • 4[3]Mate MJ et al. Nat Struct Biol, 2002; 9(6): 442
  • 5[4]Ye H et al. Nat Struct Biol, 2002; 9(9): 680
  • 6[5]Miramar MD et al. J Biol Chem, 2001; 276(19): 16391
  • 7[6]Klein JA et al. Nature, 2002; 419(6905): 367
  • 8[7]Lipton SA et al. Cell, 2002; 111 (2): 147
  • 9[8]Susin SA et al. J Exp Med, 2000; 192(4): 571
  • 10[9]Loefller M. et al. FASEB J, 2001; 15(3): 758

共引文献92

同被引文献6

  • 1Edaravone Acute Infarction Study Group.Effect of a novel free radi-cal scavenger,edaravone(MIC-186),on acute brain infarction.Randomized placebocontrolled double-blind study at multicenters. Cerebrovascular Diseases . 2003
  • 2WahlF,RenouE,MaryV,etal.Riluzolereducesbrainlesssionsandimprovesneurologicalfunctioninratsafteratraumaticbraininjury. Brain Research Bulletin . 1997
  • 3A.K. Singh,Y. Jiang.How Does Peripheral Lipopolysaccharide Induce Gene Expression in the Brain of Rats?. Toxicology . 2004
  • 4Kawasaki T,Kitao T,Nakagawa K,et al.Nitric oxide-inducedapoptosis in cultured rat astrocytes:protection by edaravone,a radi-cal scavenger. Glia . 2007
  • 5潘月星,金英,隋海娟,张智娟.脂多糖对大鼠大脑皮层星形胶质细胞分泌NO的影响[J].辽宁医学院学报,2008,29(6):484-486. 被引量:1
  • 6赵咏梅,李军泉,吕风月,闫颖,徐群渊.经侧脑室注射脂多糖诱导大鼠黑质部位小胶质细胞激活及多巴胺能神经元损伤的研究[J].中国神经免疫学和神经病学杂志,2012,19(2):121-123. 被引量:2

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部