摘要
目的建立毛细管电泳——激光诱导荧光法分离测定(R/S)-5-(2-氨基丙基)-2-甲氧基苯磺酰胺对映体。方法用异硫氰酸荧光素衍生(R/S)-5-(2-氨基丙基)-2-甲氧基苯磺酰胺,衍生产物在70mmol/L的硼砂(pH=9.5)缓冲液中用毛细管电泳分离,激光诱导荧光方法测定。结果(R/S)-5-(2-氨基丙基)-2-甲氧基苯磺酰胺对映体得到了基线分离,线性范围均为8.20×10-8～9.02×10-7mol/L。结论本方法简单,精密度高,灵敏度高,可为盐酸坦索罗辛的质量控制提供方法。
Objective To establish an HPCE-LIF method for separation and determination of the intermediate of tamsulosin hydrochloride, （R/S）-5-（2-aminopropyl）-2-methoxybenzenesulfonamide （R/S-APMB）. Methods （R）-5- （2-aminopropyl）-2-methoxybenzenesulfonamide reacted with fluorescein isothiocyanate （FITC） and then FITC-R-APMB was obtained. FITC-R-APMB was separated using 70 mmol/L borate as a buffer （pH=7.5）. The 47 cm uncoated fused-silica capillary with 75 μm internal diameter （effective length 40 cm） was used. The capillary temperature was maintained at 20 ℃. Samples were injected at a pressure of 3.45 kPa in 1 s and separated at 20 kV. Results Both R- and S-APMB had low detection limits （8.2×10^-9 mol/L）. The linear range of R- APMB was 8.20×10^-8-9.02×10^-7 mol/L. Conclusion The HPCE-LIF method may offer a simple and highly sensitive approach for quality control of tamsulosin production.
出处
《中国药物与临床》
CAS
2008年第8期597-599,共3页
Chinese Remedies & Clinics
基金
国家自然科学基金资助项目(20675063)
高校博士点专项科研基金资助项目(20060697009)
关键词
电泳
毛细管
激光诱导荧光
手性分离
盐酸坦索罗辛
中间体
荧光素
Electrophoresis,Capillary
Laser-inducedfluorescence
Chiralseparation
Tamsulosin
Intermediate
Fluorescein
