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羊水细胞培养及染色体制备方法的探讨 被引量:2

Investigation of the method about amniotic fluid cell culture and chromosome preparation
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摘要 目的:探讨羊水细胞培养及染色体制备方法。方法:孕15~29周具有产前指征的50例孕妇,抽取羊水细胞培养6~7天,换液第二天取出观察,见有许多大而亮圆的分裂期细胞时,加入秋水仙素继续培养1小时后收获、低渗、固定、制片、消化、染色。结果:50例羊水细胞培养全部成功,成功率100%。有49例获得满意的染色体分裂相。结论:该方法细胞培养成功率高、有效分裂相多、实验稳定、易于操作、成功率高。 Objective:To investigate a convenient, stable, reliable and effective method about amniotic fluid cell culture and chromosome preparation.Methods:50 prenatal women with 15-29 gestational weeks were selected from the out-patient department of our hospital,whose amniotic fluid ceils were cultured for 6-7 days.Then we observed after the second day of changing the liquid,and saw many big,bright and round dividing phase cells.Harvesting,lowwing permeability,fixing,moviemaking,digesting and staining were performed respectively after joining colchicine to continue to culture one hour. Results:50 cases of amniotic fluid cell culture were all successful,the achievement rate was 100 per cent.49 cases were satisfied with the splitting phase of the chromosome.Conclusion:We can get a high achievement rate, effective splitting phase, experimental stability, ease of operation with this cell culture method.
出处 《现代医药卫生》 2008年第15期2221-2222,共2页 Journal of Modern Medicine & Health
关键词 羊水细胞培养 染色体制备 方法 Amniotic fluid cell culture Chromosome Preparation Method
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  • 1刘晓翌,肖晓素,樊尚荣,王勇强,蔡志明.羊水细胞染色体制备方法的研究[J].中国妇产科临床杂志,2004,5(4):296-296. 被引量:20
  • 2许争峰,胡娅莉,朱瑞芳.高效羊水细胞培养技术在产前诊断中的应用[J].中华妇产科杂志,2006,41(4):275-276. 被引量:30
  • 3刘权章 韩安国 等.人类染色体方法学.绒毛染色体标本的制备方法[M].北京:人民卫生出版社,1992.280.
  • 4[3]Priest RE, Priest JH, Moinuddin JF, et al. Differentiation in human amniotic cell cultures: chronic gonadotropin production. In Vitro, 1979,15: 142-147.
  • 5Cheong KF, Knight LA, Tan M, et al. Variants of chromosome 9 in phenotypically normal individuals[J].Ann Acad Med Singapore, 1997,26(3):312-314.
  • 6刘权章.人类染色体方法学[M].北京:人民卫生出版社,1992.136.
  • 7Steele MW,Breg WR Jr.Chromosome analysis of human amniotic-fluid cells.Lancet,1966,19:383-385.
  • 8Turhan NO,Eren U,Seckin NC.Second-trimester genetic amnio-centesis:5-year experience.Arch Gynecol Obstet,2005,271:19-21.
  • 9Nassar AH,Martin D,Gonzalez-Quintero VH,et al.Genetic amniocentesis complications:is the incidence overrated? Gynecol Obstet Invest,2004,58:100-104.
  • 10Centini G,Rosignoli L,Kenanidis A,et al.A report of early (13 + 0 to 14 + 6 weeks) and mid-trimester amniocenteses:10 years' experience.J Matern Fetal Neonatal Med,2003,14:113-117.

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  • 1郭永,马振芝,苏赛,李萌萌.羊水监测[J].中国优生与遗传杂志,2005,13(3):132-132. 被引量:2
  • 2吴菊.羊水过多与胎儿妊娠结局关系探讨.中国优生优育,2008,14(2):93-94.
  • 3Mirlesse V, Duguy N, Cynober E, et al . Alphafoetoprotein and acetylcholinesterase. In amniotic fluid as a factor suggesting fetal skin and nerve lesions in a case of congenital varicella syndrome [ J ]. Prenat Diagn,2004,24(7) :498 -500.
  • 4Tones R J, Puig JG. Orphanet Hypoxanthine -guanine phoeophoribosyhr - ansferase ( HPRT ) deficiency : Lesch - Nyhan syndrome [ J ]. Rare Dis,2007,8(2) :48 -56.
  • 5Janckheere A, Carton D ,Jaeken J ,et al . Normal pregnancy outcome in L - 2 - hydroxyglutaric aciduria [ J ]. Inherit Metab Dis, 2006,29 (4) :588 -595.
  • 6Lonergan M, Aponse D, Marvin KW, et al. Tumor necrosis factorrelated apoptosis- inducing lig( TRAIL), TRAIL receptors, and the soluble receptor osteoprotegerin in human gestationl membranes and aniotic fluid during pregnancy and labor at term and preterm [J]. Clin Endocrinol Metab ,2003,88 ( 8 ) :3835 - 3839.
  • 7杨素霞 冯小春 张蓓 等.羊水细胞学检查在产前诊断中的应用.中国优生优育,2008,14(2):72-73.
  • 8Midesso V,Duguy N,Cynober E,et al.Alphafoetoprotein and acetylcholinesterase.In amniotic fluid as a factor suggesting fetal skin and nerve lesions in a case of congenital varicella syndrome[J].Prenat Diagn,2004,24(7):498 -500.
  • 9Torres RJ,Puig JG.Orphanet Hypoxanthine -guanine phoeophoribesyltr-ansferase (HPRT) deficiency:Les-ch-Nyhan syndrome[J].Rare Dis,2007,8(2):48 -56.
  • 10Jonckheere A,Carton D,Jaeken J,et al.Normal pregnancy outcome in L-2-hydroxyglutaric aciduria[J].Inherit Metab Dis,2006,29(4):588 -595.

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