摘要
目的观察益气活血复方芪丹通脉片对心肌缺血/再灌注大鼠血清白细胞介素-10(IL-10)含量的影响,探讨其心肌保护机制。方法采用结扎左冠状动脉前降支方法制备大鼠心肌缺血/再灌注模型。36只健康雄性SD大鼠,随机分为假手术组、模型组、恬尔心组及芪丹通脉片高、中、低剂量组。缺血30 min,再灌注2 h,采全血,用ELISA法检测血清IL-10含量,用HE染色观察心肌病理形态学变化,并用透射电镜观察心肌组织超微结构变化。结果芪丹通脉片高、中、低剂量组均可增加血清IL-10含量,减少炎性细胞浸润,减轻心肌超微结构损伤,其中高剂量组明显优于低剂量组,且与恬尔心组无显著差异。结论益气活血复方芪丹通脉片可促进内源性IL-10大量释放,减少炎症反应,改善受损的心肌超微结构,发挥心肌保护作用。
Objective To investigate the effects of Qi-reinforcing and blood-activating prescription, Qidan Tongrnai Tablet （ODTMT） on the IL-10 in the rat model of myocardial ischemia/reperfusion injury. A mode/of myocardial ischemia/reperfusion injury was reproduced by ligation of left anterior descending branch of the coronary artery. Thirty-six male SD rats were randomly divided into six groups （n=6）： sham operation group, model group, Diltiazem Hydrochloride group, QDTMT high-dose group, QDTMT medium-dose group and QDTMT low-dose group. After the left anterior descending branch of coronary artery was occluded for 30 minutes followed by reperfusion for 2 hours, the whole blood was collected via right common carotid artery and the serum level of IL-10 was measured by ELISA. At the same time, HE staining was performed to detect the pathological change in myocardium, and the ultrastructural alterations of rats were observed with transmission electron microscope. Results The contents of IL-10 in serum were increased in QDTMT groups. Electron microscopic examination showed that pathologic changes of myocardiocytes in QDTMT groups were milder than those of the model group. The effects in QDTMT high-dose group were better than that in the low-dose group, and has insignificant difference with Diltiazem Hydrochloride group. Conclusion Qi-reinforcing and blood-activating prescription nhibits the inflammatory reaction by facilitating the expression of endogenous IL-10 and improving myocardial ultrastructural changes after myocardial ischemia/reperfusion in rats. These actions may contribute to its protective effect on the ischemic myocardial cells.
出处
《中国中医药信息杂志》
CAS
CSCD
2008年第4期38-40,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
全军医药卫生科研重大临床专项课题(200619001)
