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金粉蕨素体外抗人卵巢癌作用研究 被引量:7

Antitumor Effect of Onychin on Ovarian Cancer Cell in Vitro
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摘要 目的探讨金粉蕨素抗人卵巢癌(COC1)细胞系细胞作用及机制。方法MTT比色法测定金粉蕨素对COC1、顺铂耐药亚株COC1/DDP及中国仓鼠卵巢(CHO-K1)细胞系细胞增殖的影响;PI染色流式细胞术(FCM)分析金粉蕨素对COC1、顺铂耐药亚株COC1/DDP及中国仓鼠卵巢(CHO-K1)细胞系细胞凋亡和细胞周期的影响。结果金粉蕨素对COC1、COC1/DDP细胞生长具有抑制作用呈剂量-效应和时间-效应关系,PI染色FCM分析发现金粉蕨素明显使COC1细胞周期停滞于G1期(G1期细胞百分数由50.3%上升到70.6%,P<0.05)。结论金粉蕨素具有体外抗人卵巢癌(COC1)细胞系细胞作用,其机制可能与细胞周期G1期阻滞有关。 Objective To study the effects and mechanism of the onychin on human ovarian cancer(COC1 ) cello. Methods MTT assay was used to determine the effect on proliferation of COC1 , COC1/ddp and CHO -K1 cell by onychin;PI stained flow eytometry (FCM) was used to analyse the apoptosis and cell eyele change of COC1 treated by Onyehin. Results Onyehin inhibited COC1 and COC1/DDP cell growth signifieantly in a dose - dependent manner and a time - dependent manner. Analysis by flow cytometry showed that onychin suppressed cell cycle progression at Gi phase. 9 ( the percentages of cells iu G1 phase rose from 50. 3% to 70.6% ). Conclusion Onyehin has anti - ovarian carcinoma effect in vitro; the mechanism might be associated with G1 phase cell cycle arrest.
出处 《医药论坛杂志》 2008年第15期4-6,共3页 Journal of Medical Forum
关键词 卵巢癌 金粉蕨素 治疗作用 WORDS Ovarian cancer Onyehin Therapeutic action
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  • 1Muggia FM. Recent updates in the clinical use of platinum compounds for the treatment of gynecologic cancers [ J ]. Semin Oncol,2004,31 ( Suppll4 ) : 17-24.
  • 2周国华,周红宇,龙远志,龙兵生,左大镒,何学贤,戈立东,高慧文,赵景涛,王宗保.SD大鼠构建慢性末端回肠炎的实验研究[J].中华消化杂志,2002,22(11):702-703. 被引量:7
  • 3Boros LG, Lee WN, Go VL, et al. A metabolic hyperchesis of cell grouth and death in pancreatic cancer [ J ]. Pancreas,2002,24( 1 ) :26-33.
  • 4Niculescu AB, Chen X, Smeets M, et al. Effects of p21 (Cipl/Wafl) at both the G1/S and the G2/M cell cycle transitions:pRb is a critical determinant in blocking DNA replication and in preventing' endoreduplication [ J ]. Mol Cell Biol, 1998,18 ( 1 ) :629-643.
  • 5Ko JC, Wang YT, Yang JL. Dual and opposing roles of ERK in regulating G1 and S - G2/M delays in A549 cells caused by hyperoxia [ J ]. Exp Cell Res, 2004,297 ( 2 ) :472-483.

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