摘要
目的：建立HPLC法测定注射用葛根素（布瑞宁）中葛根素含量的方法。方法：以30％乙醇溶液超声溶解，以HPLC外标法测定葛根素的含量，固定相为Kromasil C18色谱柱，流动相为甲醇-水（25：75），UV检测波长为250nm，流速为1．0ml／min，柱温为室温。结果：葛根素在9．66～96．60μg／ml浓度范围内浓度与吸收值成良好线性关系，r=0．9999；平均回收率为100．33％，RSD为1．15％。结论：该法简便，结果可靠，可作为布瑞宁中葛根素含量的测定方法。
OBJECTIVE To establish an HPLC method for determination of puerarin injection, and assess the concentration of puerarin injection. METHODS Used 30% alcohol to dissolve puerarin by ultrasonic wave, and puerarin was separated on Kromasil C18 reversedphase analysis column. The mobile phase was composed of methanol： wate （25： 75, V/V）. The flow rate of mobile phase was 1.0 ml/min at room temperature ; Detection wavelength was UV 250 nm. RESULTS There was a good linearity from 9.66 μg/ml to 96.60 μg/rnl （ r = 0.999 9 ） in separation of puerarin. The average recovery for puerarin injection was 100.33 %, and the relative standard deviation was 1.15%. CONCLUSION The method is simple, fast. Furthermore, the results are reliable.
出处
《天津药学》
2008年第4期26-28,共3页
Tianjin Pharmacy
