摘要
目的:探讨精索静脉曲张不育患者血清、精浆以及精子表面抗精子抗体(Antisperm Antibody,AsAb)的表达及其意义。方法:收集精索静脉曲张(Varcocele,VC)不育患者60例和非精索静脉曲张正常生育者220例,采用间接酶联免疫法(ELISA法)测定血清和精浆中AsAb,混合抗球蛋白反应试验(Mixed Agglutination Reaction Test,MARTest)检测精子表面AsAb;用计算机辅助精子分析仪测定VC组精子密度、活动精子和畸形精子百分率,并计算生育力指数。结果:①VC组血清AsAb阳性率(10%)较对照组(8.18%)相比有所增加,但差异无显著性(P>0.05);精浆AsAb阳性率16.7%、精子表面AsAb阳性率15%,较对照组(分别为7.27%和6.81%)相比,差异有统计学意义(P<0.05)。②VC不育组AsAb阳性者较AsAb阴性者相比,精液量、活动精子百分率有所下降,而畸形精子百分率增加,但差异无统计学意义(P>0.05);精子密度和生育力指数也随之降低,差异具有显著性(P<0.05)。结论:精索静脉曲张可导致AsAb阳性率增加,使生育力下降;
Objective ： To investigate the expression and its significance of antisperm antibody（AsAb） in serum, seminal plasma and the surface of sperm in male infertility cases with varicocele （VC）. Methods ： The data of 60 male infertility cases with VC and 220 subjects of healthy men （control group） were gathered for determining AsAb in serum and seminal plasma by ELISA and on surface of sperm by mixed agglutination reaction test （MARTest）. Sperm concentration, percentage of motile sperm and teratosperm were measured by computer assistant sperm analysis （CASA）and fertility indexes were calculated as well. Results： （1） The positive rate of AsAb in serum of VC cases was 10% vs . 8.18% in controls, which showed no difference（ P 〉 0.05 ）, whereas the positive rate of AsAb in seminal plasma（16.7%） and on the surface of sperm （15%） was higher than that of the control group（7.27% and 6.81%, respectively） （ P 〈 0.05）. （2）There was significant difference on semen density between the AsAb（ ＋ ） group and AsAb（ - ） group in VC infer-- tile patients, with decrease of fertility indexes in AsAb positive group （ P 〈 0.05 ）. Conclusion ： Varicocele can increase the positive rate of AsAb, and thus decreasing fertility index. Determination of AsAb in seminal plasma and on the surface of sperm is more valuable than that of mere AsAb in serum in clinical diagnosis of patients with infertility.
出处
《皖南医学院学报》
CAS
2008年第4期256-258,共3页
Journal of Wannan Medical College
基金
安徽省卫生厅重点学科科技进步基金项目(05A033)
