摘要
目的应用放射性核素骨显像评价组织工程骨修复兔股骨髁骨缺损的效果。方法取大白兔15只,抽取骨髓,行骨髓间充质干细胞分离、培养、骨向诱导。双侧股骨髁制作0.6×1.2cm的骨缺损,将诱导的成骨细胞复合珊瑚羟基磷灰石植入左侧,右侧单纯植入羟基磷灰石为对照组。术后4周、8周和l2周分别行静态核素骨显像评价骨缺损的修复能力。结果表明术后4、8、12周实验组ROI计数(单位像素)均较对照组有显著性增高(0.001)。实验组ROI计数随时间的延长呈明显的上升趋势,但术后8周始增长放缓;对照组ROI也有上升趋势,但术后8周始增长加快,均在12周达到峰值。结论骨髓间充质干细胞诱导后复合珊瑚羟基磷灰石可有效的修复股骨髁松质骨缺损。放射性核素骨显像在骨修复过程中具有动态评价血管化和骨生长的作用。
Objective To assess the effect of tissue-engineered bone graft on repairing bone defect in rabbits using radionuclide technology. Methods The fifteen healthy New Zealand rabbits were studied. Mesenchymal stem cells （MSCs） were separated and cultured form bone marrow. Cancellous bone defects（0.6×1.2cm） were created on bilateral femur condylar in rabbits. The left defects were treated with the compounds of coral hydroxyapatite ceramic（CHA） and osteoblasts which were induced and differentiated from MSCs, while the right were treated with CHA only. The counts of regions of interest（ROI） was evaluated by using radionuclide bone imaging at 4,8, 12 weeks postoperatively. Results ROI in experimental group were statistically higher than the numbers of control group at 4,8, 12 weeks postoperatively. ROI in either group increased statistically with time going-by. Eight weeks after implantation, ROI in experimental group tended to increase at lower speed, while ROI in control group had a tendency to increase at a higher speed. ROI in either group reached a peak at 12 weeks postoperatively. Conclusion The compround ofosteoblasts which were induced from MSCs and CHA is an effective method for repairing cancellous bone defects, and emission computed tomography may accurately monitor the revascularization and bone regeneration after implantation.
出处
《生物骨科材料与临床研究》
CAS
2008年第4期9-11,共3页
Orthopaedic Biomechanics Materials and Clinical Study
