摘要
建立了用反相高效液相色谱（RP～HPLC）法测定谷物中柄曲菌素（Sterigmato～cystin，ST）的含量。采用酸化乙腈震荡提取，C18固相萃取小柱净化，HPLC分离测定。样品中被测物与干扰物能得到良好的分离。柄曲菌素在10～1000μg／kg范围内与其峰面积呈良好的线性关系（r^2≥0．9999），回收率为88．4％～99．9％，相对标准偏差小于2．8％。检出限（S／N=3）为0．2ng，定量限（S／N=10）为0．5ng。该法简便、快速、准确，可用于谷物中柄曲菌素的检测。
A method was established for the determination of sterigmatocystin in cereals by reversed-phase high performance liquid chromatograph （RP-HPLC） after extraction by Ca8 cartridge. Separation and quantification were achieved by using a Agilent C18 column （250×4.6 mm i. d.,5 /lm） at 25℃, a mobile phase of acetonitrile and water（55：45） with a flow rate of 1 mL/min, and monitored with a diode-array detector at 325 nm. The linear range was from 10μg/L to 1 000μg/L with a detection limit of 10μg/L. The recoveries were 88.4%-99.9% with the relative standard deviations （RSDs） of 0.4%-2.8%. This method is simple, fast, accurate, convenient and could be used for quantitative determination of sterigmatocystin in cereals.
出处
《分析科学学报》
CAS
CSCD
2008年第4期445-448,共4页
Journal of Analytical Science
基金
国家质量监督检验检疫总局检验检疫行业标准(B107-2001)
关键词
反相高效液相色谱法
柄曲菌素
谷物
Reversed phase high performance liquid chromatography
Sterigmatocystin
Cereals
