摘要
天然产物是药物开发的重要来源，从植物中发现抗植物病原菌的活性化合物的研究受到普遍的重视，有些植物的提取物甚至可以直接用于防治作物病害。我国植物资源丰富，近年来，抗植物病原菌的植物资源的筛选是研究的热点。然而，大多数研究都是采用植物病原真菌的离体筛选方法，而对活体筛选方法，以及对采用植物病原细菌作为筛选靶标的研究较少。本文报道采用荔枝离体叶片法和利用植物病原细菌作为筛选靶标对9种传统药用植物进行抗真菌活性和抗细菌活性筛选的研究结果。
Methanol-extracts from nine plant species were screened for their bioactivity against Peronophythora litchii, Xanthomonas oryzae pv. oryzae and Ralstonia solanacearum. The plant extracts were applied on detached litchi young leaves before inoculation with sporangial suspension of P. litchii. The results showed that methanol extracts （ at conc. of 100mg dry methanol extract weight per mL） of Hamamelis mollis and Derris scabricanlis possessed the highest antifungal activity against litchi downy blight with the inhibitory effectiveness of 88.89%, followed by Phryma leptostachya （77.78%）, Clematis armcmdii （66.67%）, and Daphniphyllum macropodum （61.11%）. At the dose of lmg dry methanol extract weight, none of the tested plant methanol extracts showed inhibitory activities against X. oryzae pv. oryzae, whereas, H. mollis had the strongest antibacterial activity against R. solanacearum with the inhibitory zone diameter of 21 mm, followed by Aristolochia mollissima, D. macropodum, Pachyrhizus erosus, Vicia unijuga, and C. armcmdii with the inhibitory zone diameter between 9 to 11 mm.
出处
《植物病理学报》
CAS
CSCD
北大核心
2008年第4期 441-444,共4页
Acta Phytopathologica Sinica
基金
国家社会公益研究专项（2004DTB4J155）
天然农药与化学生物学教育部重点实验室开放基金（药重开05-11）
致谢：吉首大学张代贵老师和中国科学院武汉植物园江明喜研究员帮助鉴定植物种类,特致谢忱.
