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一种高效可直接用于PCR扩增的不吸水链霉菌基因组DNA的提取方法 被引量：2

A Hi-Effect Extraction Method Used Directly for PCR to Genome DNA of Streptmyces Ahygroscopicus
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摘要 通过对提取不吸水链霉菌基因组DNA的冻融法、微波法和溶菌酶破壁法等几种方法进行对比、分析,得出结论:溶菌酶破壁法所提取的不吸水链霉菌基因组DNA可直接用于PCR扩增。这为今后不吸水链霉菌相关基因的实验研究提供了可靠的理论依据。 The extraction of Genome DNA of Streptmyces Ahygroscopicus were compared and analyzed through the methods of freeze-thaw,microwave and break-shell of lysozyme. The results showed that the Genome DNA of Streptmyces Ahygroscopicus extraction through the method of the break-shell of lysozyme can be used directly for PCR. The study gave a theoretical basis for the future lab research on relative gene of Streptmyces Ahygroseopicys.
作者 吴红艳 陈飞 桓明辉 关艳丽 郭玲玲
机构地区 辽宁省微生物科学研究院
出处 《生物技术通报》 CAS CSCD 2008年第4期195-197,202,共4页 Biotechnology Bulletin
关键词 不吸水链霉菌 基因组DNA 提取 PCR扩增 Streptmyces ahygroscopicus Genome DNA Extraction PCR
分类号 S476 [农业科学—农业昆虫与害虫防治] Q78 [生物学—分子生物学]
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共引文献296

同被引文献13

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生物技术通报
2008年 第4期

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