摘要
以12个辣椒品种为材料,采用顺序提取法(重蒸水5、%NaCl和0.2%NaOH)提取种子贮藏蛋白(水溶蛋白、盐溶蛋白和碱溶蛋白),利用SDS-PAGE电泳技术,对辣椒种子贮藏蛋白质的组成进行了分析。结果表明:辣椒种子水溶蛋白(19条带)、盐溶蛋白(24条带)及碱溶蛋白(24条带)的种类数量差异较小,但分布区域和相对含量有很大差异,盐溶蛋白的相对含量最多,占贮藏蛋白的44.4%;其次是水溶蛋白,占37.5%;碱溶蛋白最少,只占18.1%。从38.8 KD到55.6 KD是水溶蛋白的主区,从17.5 KD到35.6 KD是盐溶蛋白的主区,从8.3 KD到39.9 KD是碱溶蛋白的主区。
Seed storage proteins of 12 capsicum cultivars were respectively extracted using distilled H2O, 5% NaC1 and 0.2% NaOH. Systemic analysis of the composition of storage proteins of capsicum seeds was done by the application of SDS-PAGE electrophoresis techniques. The results showed that amount of protein strips were 19 （water-soluble pro- teins） ,24 （salt-soluble proteins）,and 24（alkali-soluble proteins）. The most of percentage distributing were salt-soluble proteins（44.4%）, water-soluble proteins（37.5%）, and alkali-soluble proteins （18.1%）. The main area of protein components were 38.8-55.6 KD（water-soluble proteins）, 17.5-35.6 KD（salt-soluble proteins） ,and 8.3-39.9 KD （alkali -soluble proteins）.
出处
《北方园艺》
CAS
北大核心
2008年第7期1-4,共4页
Northern Horticulture
基金
国家级星火计划资助项目(2004EA760041)
