摘要
应用兔大脑中动脉阻断模型，分离出突触膜（synapticplasmamembrane，SPM），线粒体（mitochondria，Mit）并测其Na+－K+－ATP酶活性，同时以放免法测定脑组织内皮素－1（ET－1）含量。结果发现脑缺血后脑组织ET－1含量显著增加，SPM、MitNa+－K+－ATP酶活性下降，两者呈显著负相关（r1＝－0．9776，P＜0．05；r2＝－0．9980，P＜0．02）．提示ET－1在脑缺血脑水肿中的作用可能与其抑制Na+－K－－ATP酶活性有关。
In the model of rabbit middle cerebral artery occlusion (MCAO), the synaptic plasma membrae (SPM), and mitochondria (Mit) were dissected and the activity of Na+ -K+ - ATPase in them and the contents of ET - 1 in brain tissue were determined. The content of ET - 1 was significantly increased and the activity of Na+-K+ - ATPase in SPM and Mit were decreased after cerebral ischemia, and there was negative correlation between content of ET- 1 and the activity of Na+-K+ - ATPase in SPM and Mit (r1= -0.9776, P<0.05;r,= -0.9980,P<0.02 respectively). These results suggested that the effect of ET - 1 on ischemic brain edema was associated with its inhibition of Na+ - K+ - ATPase activity in SPM and Mit.
出处
《上海第二医科大学学报》
CSCD
1997年第6期427-429,共3页
Acta Universitatis Medicinalis Secondae Shanghai
基金
国家卫生部八五攻关脑缺血脑水肿课题!85-915-03-08
关键词
脑缺血
内皮素-1
线粒体
突触膜
ATP酶
cerebral ischemia
endothelin-1
mitochodria
synaptic plasma membrane
Na^+ -K+ -ATPase
