摘要
目的：检测宫颈癌患者血浆游离DNA含量并探讨其与临床病理特征之间的关系。方法：收集42例宫颈癌、20例宫颈鳞状上皮内瘤样病变Ⅲ患者和20例健康妇女的外周血,用微量基因组提取试剂盒提取血浆DNA,用双链DNA高敏试剂盒和荧光仪测定血浆DNA含量。结果：宫颈癌患者的血浆DNA水平（15.14±7.10）ng/mL明显高于宫颈鳞状上皮内瘤样病变Ⅲ（8.10±3.06）ng/mL和正常对照组（7.60±3.87）ng/mL（P〈0.05）,宫颈鳞状上皮内瘤样病变Ⅲ和正常对照组之间差异无统计学意义（P〉0.05）。Ⅰ期宫颈癌患者的血浆DNA水平（12.78±5.58）ng/mL明显高于正常和宫颈鳞状上皮内瘤样病变Ⅲ组（P〈0.05）,Ⅱ-Ⅲ期患者的血浆DNA水平（17.99±7.81）ng/mL明显高于Ⅰ期组（P〈0.05）。Ⅰ-ⅡA期患者中,肿块直径〉4 cm者的血浆DNA水平（15.60±6.13）ng/mL明显高于肿块直径〈4cm者（9.68±2.84）ng/mL（P〈0.05）。以正常对照者血浆DNA水平的95%可信区间上限15.70 ng/mL为界值,其诊断的敏感性为38.10%,特异性为92.50%,阳性预测值为84.21%,阴性预测值为58.73%。结论：宫颈癌患者的血浆DNA水平与疾病的发生、发展和预后密切相关,血浆DNA水平可能有助于宫颈癌的鉴别诊断。
Objective To determine the plasma DNA level and investigate its clinicopathological significance in cervical cancers.Methods Blood samples were collected from 42 patients with cervical cancer,and 20 patients with CIN Ⅲ and 20 healthy women.Plasma DNA was extracted by commercial kit and detected by fluorescntmeter.Results The mean plasma DNA level of the cervical cancer patients was（15.14±7.10）ng/mL,significantly higher than that of the CIN Ⅲ patients [（8.10±3.06）ng/mL] and normal controls[（7.60±3.87）ng/mL]（P〈0.05）.There was no statistic difference of the plasma DNA level between the CIN Ⅲ and normal group（P〉0.05）.The mean plasma DNA level of the patients with CIN Ⅲ was（12.78±5.58）ng/mL,significantly higher than that of the patients with CIN Ⅲ and normal controls（P〈0.05）,and significantly lower than that of the stage Ⅱ～Ⅲ patients [（17.99±7.81）ng/mL]（P〈0.05）.The mean DNA level of the patients with tumor diameter larger than 4 cm was（17.99±7.81）ng/mL,significantly higher than that of the patients with tumor diameter less than 4 cm[（9.68±2.84）ng/mL]（P〈0.05）.When the cut-off for diagnosis of cervical cancer was 15.70 ng/mL,the sensitivity and specificity and positive predictive value and negative predictive value were 38.10%,92.50%,84.21% and 58.73%,respectively.Conclusion Plasma DNA level is closely related with the malignant transformation, development and prognosis of cervical cancer.It may have a potential clinical use as a differential diagnosis indicator in cervical cancer.
出处
《中华实用诊断与治疗杂志》
2008年第8期576-578,共3页
Journal of Chinese Practical Diagnosis and Therapy
基金
广东省自然科学基金资助项目(编号:5000123)
关键词
宫颈癌
血浆
游离DNA
Cervical cancer, plasma, free DNA
