摘要
应用聚合酶链反应（ＰＣＲ）扩增载脂蛋白Ｅ（ａｐｏＥ）基因后，ＰＣＲ产物用限制性内切酶ＨｈａＩ酶解后进行聚丙烯酰胺凝胶电泳。共检测了１５８例广西壮族健康人群的ａｐｏＥ基因型，根据表型分布计算出等位基因频率分布，并与１２０例汉族健康人群对比。结果ＡｐｏＥ基因频率分布壮族为ε４０．０７９，ε３０．８１３，ε２０．１０８，汉族为ε４０．０６３，ε３０．８３３，ε２０．１０４，两组人群ａｐｏＥ基因频率分布基本相同。
To get gene frequency distribution of apolipoprotein E(apo E)in Zhuang Nationality population,158 healthy people of Guangxi Zhuang Nationality were investigatedThe apoE gene sequence was amplified in polymerase chain reaction (PCR) and the PCR products were digested by restriction endonuclease HhaI and electrophoresisedThen allele gene frequency of the population were calculated according to phenotype distributionThe results showed that the apo E gene frequency distribution of Zhuang Nationality were ε40079,ε30813,ε20108,while the Han’s were ε40063,ε30833,ε20104The difference of the two population wasn’t significant
出处
《广西医学》
CAS
1997年第6期982-984,共3页
Guangxi Medical Journal
基金
广西区卫生厅青年基金
