摘要
采用错配PCR—RFLP法，检测8个品种314头猪IGF-2基因内含子3的3072位点多态性及其在群体中的分布。结果表明：引入错配碱基构造出新的酶切位点，在很大程度上降低了试验成本且不影响基因型判定结果。IGF-2基因内含子3的3072位点（G→A）变异多态在猪群中分布差异极显著。国外品种猪中等位基因A的频率高于我国地方品种猪，我国地方品种猪（二花脸猪、梅山猪、五指山猪、淮猪）群体中绝大多数为GG基因型；而国外品种猪（长白猪、大约克猪、杜洛克猪）群体中GA和AA基因型频率分别为60％-70％和20％～30％。由杜洛克猪与二花脸猪、梅山猪杂交育成的苏太猪，其群体中GA基因型频率接近78％，大大超过地方品种猪。
A mispairing PCR was used to flank the paternal expressed IGF-2 gene intron 3 G3072A mutation in 314 pigs. The results showed that the mispairing PCR was a rapid, simple and efficient method to detect single nucleotide polymorphism （SNP）. The single nucleotide polymorphism （G→A transition） in IGF-2 gene intron 3 3072 site was significantly different between the imported populations and Chinese native populations （P 〈 0. 01 ）. The A allele frequencies of external breeds were higher than the local breeds. GA and AA genotypes in imported populations （Landrace, Large White and Duroc pigs） reached 60% - 70% and 20% - 30% , respectively. Sutai pigs from Landrace × Large White × Erhualian cross frequencies of GA genotype had reached 78%, and were the significantly higher than Chinese native populations.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2008年第2期48-52,共5页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
江苏省高新技术研究计划项目(BG2004317,BG2005304)
