摘要
[目的]建立番荔枝内酯类化合物squamocin和bullatacin的液相色谱串联质谱(LC-MS/MS)检测方法,并测定其在大鼠肝微粒体中的酶动力学参数。[方法]建立微粒体孵育体系,启动反应后测定不同时间孵育体系内剩余的squamocin和bullatacin的浓度,用底物消除法计算其酶动力学参数。[结果]在大鼠肝微粒体中,squamocin和bullatacin的表观米氏常数KM值分别为(1.6±0.5)和(2.0±0.4)μmol/L。[结论]该方法适用于squamocin和bullatacin的酶动力学常数的测定。
[Objective] This study established a LC-MS/MS method for the determination of squamocin and bullatacin, and calculated their enzyme kinetics in rat microsome. [Method] Established microsome incubation system, determined the remaining concentration of substrate at different time points after starting the reaction, and calculated enzyme kinetic parameters with substrate depletion method. [Result] In rat microsome, the apparent KM of squamocin and bullatacin were （1.6±0.5） μmol/L and（2.0±0.4） μmol/L.[Conclusion] This method was suitable for the enzyme kinetic study of squamocin and bullatacin.
出处
《安徽农业科学》
CAS
北大核心
2008年第21期9076-9078,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金资助项目(30572253)
