摘要
目的:观察蛋清溶菌酶(HEWL)对人乙型肝炎病毒(HBV)的抑制作用。方法:以2.2.15细胞为模型,以苦参碱为阳性对照药,采用细胞病变法(CPE)和酶联免疫吸附实验(ELISA)判定溶菌酶对2.2.15细胞乙型肝炎表面抗原(HBsAg)和乙型肝炎e抗原(HBeAg)表达水平的抑制效果。结果:HEWL对细胞的最大无毒浓度(TC0)和半数中毒浓度(TC50)均>1 000μg.mL-1,HEWL对2.2.15细胞HBsAg抑制作用的半数有效浓度(IC50)为(213.0±71.3)μg.mL-1,对细胞HBeAg无抑制作用。苦参碱对2.2.15细胞的TC0和TC50均>250μg.mL-1。苦参碱对2.2.15细胞HBsAg抑制作用IC50的为(54.2±17.1)μg.mL-1,对HBeAg无抑制作用。结论:蛋清溶菌酶对2.2.15细胞的HBsAg表达有较明显的抑制作用,对其HBeAg无抑制作用。
Objective： To examine the antiviral effect of hen egg-white lysozyme （HEWL） against hepatitis B virus （HBV）. Methods：The inhibitory activity of HEWL against HBV was tested in vitro on 2.2.15 cells, using matrin as control. Data elaboration was carried out by calculation of the cytopathic effect （CPE）and ELISA. Results：The maximum nontoxic con- centration （TCO） and the 50% toxic concentration （TC50） of HEWL on 2.2.15 cell were all 〉 1000 μg·mL-1, the 50% inhibitory concentration （IC50） of HEWL to HBsAg was （213.0 ±71.3）μg·mL-1, HEWL had no effect on HBeAg. The TCO and TCS0 of matrin were all 〉 250 μg·mL-1 , the 50% inhibitory IC50 of matrin to HBsAg was （54.2 ± 17.1 ）μg·mL-1, ma- trin also had no effect on HBeAg. Conduslon：HEWL can inhibit the activity of HBV by inhibiting the expression of HBsAg, but has not effect on HBeAg.
出处
《军医进修学院学报》
CAS
北大核心
2008年第4期277-279,共3页
Academic Journal of Pla Postgraduate Medical School
