摘要
目的通过动物实验,观察、了解置入脂肪垫前后模型兔膝骨关节炎滑膜组织中超氧化物歧化酶(SOD)、丙二醛(MDA)及一氧化氮(NO)水平的变化,探讨"CHK"术式治疗髌股关节骨性关节炎前后自由基水平的变化,从而了解其生化方面的一些机理,为临床应用提供一定的客观依据。方法采用鬼头康彦的造模方法,36只健康大白兔,随机分为A、B、C 3组,每组12只。A组为空白对照组(不做任何处理),B组为模型组,C组为实验组(施行"CHK"术式)。将3组动物于术后第3周、6周、9周分批处死,每组处死4只(8膝),取其膝关节滑膜组织制成质量分数为10%的组织匀浆(每组8个标本),检测其SOD、MDA及NO的水平,将所得数据进行比较。结果(1)空白对照组各时期膝关节滑膜组织匀浆中SOD、MDA及NO的含量均无显著差异。(2)与空白对照组比较,模型组滑膜组织匀浆中SOD的含量明显降低;MDA和NO的含量则随观测时间的递增而急剧升高,且二者的含量于造模成功后第9周达到较高水平。(3)与模型组比较,实验组滑膜组织匀浆中SOD的含量随观测时间的递增而逐渐升高,于术后第9周与空白对照组相比差距达到最小;MDA和NO的含量随观测时间的递增而逐渐降低,于术后第9周与空白对照组相比差距达到最小。结论"CHK"术式能在一定程度上升高兔膝OA滑膜组织中SOD的含量、降低MDA和NO的含量,阻止自由基对兔膝关节软骨及周围组织的继续损伤,为局部组织修复创造有利条件,从而有效治疗兔膝OA,为临床治疗膝关节骨性关节炎提供了客观的实验室依据。
Objective To observe and find out the changes of SOD, MDA and NO in synovial tissues of the rabbit＇s knee osteoarthritis models before and after implanting the pedicled infrapatellar fat pad by using animal experiment, and discuss the changes of free radical before and after the ＂CHK＂ surgical treatment upon its, for the purpose of knowing it＇s mechanism in biochemistry and proposing some objective basis for clinical application. Methods Thirty-six white rabbits with big ears were divided into A, B and C three groups randomly, every group with twelve rabbits. Group A was normally fed as the blank control group （received no treatment）, group B was the model group, and group C was the experimental group （namely performed the operative method of the ＂CHK＂）. All animals were sacrificed in batch at week 3, 6 and 9 after operation, and four rabbits of both groups were killed respectively. Their synovial tissues of the knee were adopted to be cranked out the tissue homogenate with 10% mass fraction. Finally, checked out the levels of SOD, MDA and NO in such tissue homogenate and the data were compared to direct sequencing. Results There is no significant difference about these contents of SOD, MDA and NO in synovial tissue homogenate of the knee joint of the blank control group in various periods. The SOD content in synovial tissue homogenate of the model group decreased markedly contrasting to the blank control group; the contents of MDA and NO elevated rapidly along with increasing time of observation and arrived at the higher level at week 9 after making model successfully. The SOD content in synovial tissue homogenate of the experimental group increased gradually along with increasing time of observation contrasting to the model group and the gaps between the experimental group and the blank control group achieved the minimum at week 9 after operation; the contents of MDA and NO reduced gradually along with increasing time of observation and the gaps between the two groups also attained the minimum at week 9 after operation. Conclusion The ＂ CHK＂ surgical treatment can rise the contents of SOD, MDA and NO in synovial tissues of the rabbit ＇s knee osteoarthritis, to some extent, preventing continuing injury of cartilage in articular genus and surrounding tissues in rabbits by reactive free radicals and promoting tissue repair, so it has remarkable effect, for the purpose of proposing some objective basis for clinical application.
出处
《贵州医药》
CAS
2008年第8期680-684,共5页
Guizhou Medical Journal
