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绝经后退行性脊柱滑脱椎体软骨终板内雌激素受体表达与青年女性的差异 被引量：3

Disparity of the expression of estrogen receptor in cartilaginous endplate between patients with postmenopausal degenerative spondylolishesis and young women
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摘要 目的:探讨雌激素受体(ER)在绝经后退行性脊柱滑脱(DS)软骨终板内的表达及其意义。方法:用RT-PCR方法检测绝经后DS与绝经前年轻女性正常腰椎(N)的软骨终板内ER mRNA的表达;Western blot方法检测绝经后DS与N的软骨终板内ER蛋白质的表达;用免疫组织化学法(Envision法)检测绝经后DS与N的软骨终板内ER的分布和表达,测定绝经后DS患者血液中雌激素浓度;分析雌激素、ER和DS之间的关系。结果:ER mRNA的表达量在绝经后DS的软骨终板中较N明显下降(P<0.05);绝经后DS的软骨终板中ER蛋白质的表达量较N明显下降(P<0.05);免疫组织化学结果显示ER在绝经后DS的软骨终板中仅少量表达,在N的软骨终板中表达量较强;绝经后DS患者血液中雌激素浓度明显下降。结论:女性在绝经后体内雌激素下降,软骨终板中ER表达量较N显著下调,可能是引起绝经后女性DS高发的原因之一。 Objective To investigate the nous endplate of patients with postmenopausal expression pattern and significance of estrogen receptor （ER） in cartilagidegenerative spondylolishesis （DS）. Methods RT-PCR was used to detect the expression of ER mRNA in cartilaginous endplate of postmenopausal DS and before-menopausal young female lumbar vertebra （N）. The expression of ER protein in cartilaginous endplate of postmenopausal DS and N were measured by Western blot. Immunohistochemistry （Envision method） was carried out to examine the location and expression of ER in cartilaginous endplate of postmenopausal DS and N, detect the estrogen concentration in blood of the patients, then the relationships among the estrogen, the expression pattern of ER and DS were analyzed. Results The expression of ER mRNA in cartilaginous endplate of postmenopausal DS was obviously lower than in cartilaginous endplate of N （P 〈 0. 05 ）. Comparing with the N, the expression of ER protein increased significantly in cartilaginous endplate of postmenopausal DS （P 〈 0.05 ）. Only low expression of ER was detected in cartilaginous endplate of postmenopausal DS, but the expression was much higher in N. Comparing with the young women, the estrogen concentration of the postmenopausal patients in blood was obviously low. Conclusions The blood estrogen concentration of the patients is obviously low after menopause. The expression of ER in cartilaginous endplate of postmenopausal DS obviously down-regulates compareing with the N,implying its possible role in the pathogenesis, pathology and development of degenerative spondylolishesis
作者 徐小彬 吴小涛 王运涛 隋吉生
机构地区 东南大学临床医学院 东南大学附属中大医院骨科
出处 《东南大学学报（医学版）》 CAS 2007年第5期344-348,共5页 Journal of Southeast University(Medical Science Edition)
关键词 雌激素受体 退行性脊柱滑脱 软骨终板 estrogen receptor degenerative spondylolishesis cartilaginous endplate
分类号 R681.5 [医药卫生—骨科学]
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参考文献17

  • 1HA K Y, CHANG C H, KIM K W, et al. Expression of estrogen receptor of the facet joints in degenerative spondylistheis [ J ], Spine ,2005,30 (5) :562-566.
  • 2BODEN S D,RIEW K D,YAMAGUCHI K,et al. Orientation of the lumbar facet joints:association with degenerative disc disease[ J]. J Bone Joint Surg Am. 1996.78 :403-411.
  • 3SATO K,WAKAMATSU E,YOSHIZUMI A,et al. The configuration of the laminas and facet joints in degenerative spondylolisthesis., a clinicoradiologic study [ J]. Spine, 1989, 14: 1265-1271.
  • 4LOVE T W,FAGAN A B,FRASER R D. Degenerative spon- dylolisthesis,developmental or acquired? [ J ]. J Bone Joint Surg Br, 1999.81:670-674.
  • 5RICHMOND R S,CARLSON C S, REGIATER T C ,et al. Functional estrogen receptors in adult articular cartilage:estrogen replacement therapy increases chondrocyte synthesis of proteoglycans and insulin-like growth factor binding protein [ J ]. Arth Rheum, 2000,43 ( 9 ) : 2081-2209.
  • 6乐杰.妇产科学[M]5版[M].北京：人民卫生出版社,2002.116 - 118.
  • 7韩德韬,张发惠.椎间盘椎间孔和椎管间生物力学相互关系的实验研究[J].中华外科杂志,1989,27(10):581-584. 被引量：24
  • 8IMADA K, MATSUI H,TSUJI H. Oophorectomy predisposes to degenerative spondylolisthesis[ J]. J Bone Joint Surg Br,1995, 77:126-130.
  • 9YVES M B, MARK P B, RAYMOND G, et al. Intervertebral disc height in treated and untreated overweight post-menopausal women[ J]. Human Repr,2005,20( 12 ) :3566-3570.
  • 10万荣,杨庆铭,邓廉夫,张玥.雌激素对软骨细胞胶原表型表达的影响[J].中国运动医学杂志,2005,24(2):187-190. 被引量：7

二级参考文献33

  • 1[1]Felson DT, Zhang Y. An update on the epidemiology of knee and hip osteoarthritis with a view to prevention. Arthritis Rheum, 1998, 41(8): 1343-1355.
  • 2[2]Nevitt MC, Cummings SR, Lane NE, et al. Association of estrogen replacement therapy with the risk of osteoarthritis of the hip in elderly white women study of osteoporotic fractures research group. Arch Intern Med, 1996, 156(18): 2073-2080.
  • 3[3]Zhang Y, McAlindon TE, Hannan MT, et al. Estrogen replacement therapy and worsening of radiographic knee osteoarthritis: the framingham study. Arthritis Rheum, 1998, 41(10): 1867-1873.
  • 4[4]Turner AS, Athanasiou KA, Zhu CF, et al. Biochemical effects of estrogen on articular cartilage in ovariectomized sheep. Osteoarthritis Cartilage, 1997, 5(1):63-69.
  • 5[5]Da.Silva.JA, Larbre JP, Seed MP, et al. Sex differences in inflammation induced cartilage damage in rodents: The influence of sex steroids. J Rheumatol, 1994, 21(2): 330-337.
  • 6[6]Tsai CL, Liu TK. Estradiol.induced knee osteoarthrosis in ovariectomized rabbits. Clin Orthop, 1993,2(1):295-302.
  • 7[7]Tsai CL, Liu TK. Up.regulation of estrogen receptors in rabbit osteoarthritic cartilage. Life Sci, 1992, 50(22):1727-1735.
  • 8[8]Tsai CL, Liu TK. Inhibition of estradiol.induced early osteoarthritic changes by tamoxifen. Life Sci, 1992, 50(25): 1943-1951.
  • 9[9]Poole AR, Rosenberg LC, Reiner A, et al. Contents and distributions of the proteoglycans decorin and biglycan in normal and osteoarthritic human articular cartilage. J Orthop Res, 1996, 14(5):681-689.
  • 10[10]Cs.Szabo G, Roughley PJ, Plaas AH, et al. Large and small proteoglycans of osteoarthritic and rheumatoid articular cartilage. Arthritis Rheum, 1995, 38(5):660-668.

共引文献182

同被引文献42

  • 1陈辉,吴小涛,王运涛,茅祖斌,王宸,洪鑫,王以进.腰椎后柱“骨-腱-骨”结构的完整性对邻近节段生物力学的影响[J].现代医学,2007,35(6):454-457. 被引量：10
  • 2张宏军,范顺武,方向前,赵凤东,刘叶,马江涛.腰椎退变与不稳的影像学研究[J].中国临床解剖学杂志,2006,24(3):279-282. 被引量：13
  • 3JACOBSEN S, SONNE- HOLM S, ROVSING H, et al. Degener- ative lumbar spondylolisthesis: an cpidemiological perspective [ J ]. Spine,2007,32 ( 1 ) : 120-125.
  • 4HASEGEWA K, KITAHARA K, HARA T, et al. Biomechanical evaluation of segmental instability in degenerative lumbar sDondvlolisthesis [J]. Eur Soine J. 2009, 18 ( 4 ) :465-470.
  • 5PFIRRMANN C W, METZDORF A, ZANETTI M, et al. Mag- netic resonance classification of lumbar intervertebral disc de- generation [ J ]. Spine, 2001,26 ( 17 ) : 1873-1878.
  • 6CHEN I R, WEI T S. Disc height and lumbar index as inde- pendent predictors of degenerative spondylolisthesis in middle-aged women with low back pain [ J ]. Spine, 2009,34 ( 13 ) : 1402-1409.
  • 7NAGAOSA Y, KIKUCHI S, HASUE M, et al. Pathoanatomie mechanisms of degenerative spondylolisthesis. A radiographic study [ J ]. Spine, 1998,23 ( 13 ) : 1447-1451.
  • 8ADAMS M A, MCNALLY D S, DOLAN P. Stress distributions inside intervertebral discs, the effects of age and degeneration [J]. J Bone Joint Surg (Br) ,1996,78 (6):965-972.
  • 9FUJIWARA A,TAMAI K,AN H S, et al. The relationship be- tween disc degeneration,facet joint osteoarthritis,and stability of the degenerative lumbar spine [ J ]. J Spinal Disord,2000, 13 (5) :444-450.
  • 10DAI L Y. Orientation and tropism of lumbar facet joints in de- generative spondylolisthesis [ J ]. Int Orthop, 2001,25 ( 1 ) : 40-42.

引证文献3

二级引证文献4

东南大学学报（医学版）
2007年 第5期

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