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不同发育时期大鼠肠道黏膜形态与树突状细胞发育活化的研究 被引量:4

Morphology and ontogeny of intestinal dendritic cells of rats in different developmental stages
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摘要 目的探讨不同发育时期SD大鼠肠道黏膜形态发育及树突状细胞发育与活化的关系。方法25只SD大鼠随机分为五组,分别在3、5、7、9、11周应用图像分析检测技术及流式细胞技术检测幼鼠肠黏膜绒毛与肠集合淋巴结发育状况及肠树突状细胞发育及活化指标。结果不同发育时期肠绒毛长度、绒毛宽度、绒毛面积、隐窝深度及绒毛长度/隐窝深度差异均有统计学意义(P<0.05);不同发育时期小肠集合淋巴结面积增加差异有统计学意义(P<0.05);随着周龄的增加,肠道OX62^+总树突状细胞增加,OX62^+/CD4^-/OX41^-亚型树突状细胞减少,同时,OX62^+/CD4^+/OX41^+亚型树突状细胞增加,变化均有统计学意义(P<0.05)。结论随着周龄的增长,SD大鼠小肠绒毛-隐窝轴增殖分化,肠道树突状细胞逐渐发育与成熟,且两者的发育成熟状况基本同步。SD大鼠肠黏膜形态及黏膜免疫细胞早在断乳期就逐渐成熟,在7~9周时发育幅度最大,并在11周时逐渐达到成熟状态。断乳早期,食物改变会影响SD大鼠肠道黏膜形态发育及树突状细胞发育活化。 Objectives To explore the relationship of the intestinal morphology and the dendritic cells (DC) ontogeny in Spragne-Dowly rats in different developmental stages. Methods Neonatal rats were randomly divided into 5 groups including G3w, GSw, G7w, G9w and Gllw. Using morphometric analysis and flow eytometry analysis, the de- velopment of villous-crypt axis in the small intestine and Peyerls patches (PP) were studied. Results (1) The morphometry parameters of villous-crypt axis in the small intestine, such as villous height, villous width, villous areas, crypt depth and the ratio of villous height and crypt depth changed significantly in different developmental stages (P 〈 0.05). (2) The levels of the area of Peyerls patch were increased significantly (P 〈 0.05). (3) With the age in- creased the number of OX62+DCs and the number of OX62+CD4+SIRP+DC subsets increased, whereas the number of OX62+CD4+SIRP-DC decreased. Conclusions (1) With the age increased, the villous-crypt axes proliferated and differentiated in the small intestine, simultaneously, there were also developments and maturations in PP DCs phenotypic expression of rat. (2) The levels of the morphometry parameters of the small intestine and the development of immune cells (especially DC) increased from the early weaning period, peaked in the postnatal 7+ week and reached a rela- tively mature state in the 11+ postnatal week. (3) In the early weaning period, the number and the phenotypic ex- pression of PP DCs as well as the morphological changes rapidly, due to ingestion of the solid food. Detailed mechanism needs to be studied further.
出处 《临床儿科杂志》 CAS CSCD 北大核心 2008年第8期708-711,共4页 Journal of Clinical Pediatrics
基金 国家自然基金资助项目(No.30571979)
关键词 肠黏膜 树突状细胞 肠集合淋巴结 肠道发育 intestinal mucosa dendritic cell Peyer's- Patches intestinal development
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  • 1Shreedhar VK, Kelsall BL, Neutra MR. Cholera toxin induces migration of dendritic cells from the subepithelial dome region to T- and B-cell areas of Peyer's patches [J]. Infect Immun 2003,71 (1):504-509.
  • 2Uhlig HH, Powrie F. Dendritic cells and the intestinal bacterial flora:a role for localized mucosal immune responses [J]. Clin J Invest,2003,112(5) :693-706.
  • 3Brenan M. Rees DJ. Sequence analysis of rat integrin alpha E1 and alpha E2 subunits:tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph [J]. Eur J Immunol, 1997,27( 11 ) :3070-3079.
  • 4Liu L, Zhang M, Jenkins C,et al. Dendritic cell heterogeneity in vivo:two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression [J]. J Immunol, 1998,161 (3) : 1146- 1155.
  • 5Bilsborough J, George TC, Norment A, et al. Mucosal CD8alpha'DC,with a plasmacytoid phenotype,induce differentiation and support function of T cells with regulatory properties [J ]. Immunology, 2003,108 (4) : 481-492.
  • 6Zaph C, Troy AE, Taylor BC, et al. Epithelial-cell-intrinsic IKK-beta expression regulates intestinal immune homeostasis [J]. Nature,2007,446(7135) :552-556.
  • 7顾宪红.断奶仔猪小肠结构和功能的变化及其影响因素[J].中国饲料,1999(1):27-29. 被引量:15

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  • 1Katri Peuhkuri,Heikki Vapaatalo,Riitta Korpela.Even low-grade inflammation impacts on small intestinal function[J].World Journal of Gastroenterology,2010,16(9):1057-1062. 被引量:9
  • 2Kvistgaard AS Pallesen LT, Arias CF, et al. Inhibitory effects of human and bovine milk constituents on rotavirus infections[J]. J Dairy Sei, 2004, 87 (12): 4088-4096.
  • 3Cavatorta D J, Erb HN, Flaminio MJ. Ex vivo generation of mature equine monocyte -derived dendritic cells[J]. Vet Immunol Immunopathol, 2009, 131 (3): 259-267.
  • 4Miyasaka K, Hanayama R, Tanaka M, et al. Expression of milk fat globule epidermal growth factor 8 in immature dendritic cells for engulfment of apoptotic cells [J], Eur J Immunol, 2004, 34 (5): 1414-1422.
  • 5Raymond A Ensslin MA, Shur BD. SED1/MFG-E8: A bi-motif protein that orchestrates diverse cellular interactions[J]. J Cell Biochem, 2009, 106 (6): 957-966.
  • 6Petersona JA, Patton S Hamoshc M. Glycoproteins of the human milk fat globule in the protection of the breast-fed infant against infections [J]. Biol Neonate, 1998, 74 (2): 143-162.
  • 7Akakura S, Singh S, Spataro M, et al. The opsonin MFG-E8 is a ligand for the alphavbeta5 integrin and triggers DOCK180-dependent Racl activation for the phagocytosis of apoptotic cells [J]. Exp Cell Res 2004, 292 (2): 403--416.
  • 8Clayton A, Court J, Navabi H, et al. Analysis of antigen presenting cell derived exosomes, based on immuno-magnetic isolation and flow cytometry [J]. J Immunol Methods, 2001, 247 (1/2): 163-174.
  • 9Morelli AE, Larregina AT, Shufesky W J, et al. Endocytosis, intracellular sorting, and processing of exosomes by dendritic cells[Jl. Blood, 2004, 104 (10): 3257-3266.
  • 10Oshima K, Aoki N, Negi M, et al. Lactation dependent expression of an mRNA splice variant with an exon for a multi- ply O-glycosylated domain of mouse milk fat globule glycoprotein MFG-E8 [J]. Biochem Biophys Res Commun, 1999, 254 (3): 522-528.

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