摘要
目的：提高梅苏颗粒质量标准。方法：采用薄层色谱法分别对方中乌梅肉、紫苏叶、薄荷进行定性研究，采用高效液相色谱法，以乙腈-水（11：89）为流动相，C18色谱柱，检测波长为250nm，测定方中葛根素的含量。结果：TLC鉴别分离度好、专属性强；葛根素在0．0660～0．3301μg线性关系良好，r=1，加样回收率为99．36％，RSD=0．64％（n=6）。结论：所建立的质量标准方法可靠、准确、专属性强，可有效控制梅苏颗粒的质量。
Objective： To improve the quality standard for Meisu granule. Methods： The TLC method was used for the qualitative identification of fmctus mume, folium perillae, herba menthae in the formula. The content of puerarin was determined by an HPLC method with a C18 column and a mobile phase of acetonitrile-water （11：89）, the detection wavelength was 250nm Results： The characteristic identification by TLC was distinct and highly specific. Puerarin showed a good linear relation ship at a range of 0. 006 0 --0. 3301μg （ r = 1 ） and average recovery was 99.36%, RSD =0. 64% （n = 6） . Conclusion： The method is reliable, accurate and specific, and can be used for the quality control of Meisu Granule.
出处
《中国现代中药》
CAS
2008年第8期23-25,31,共4页
Modern Chinese Medicine
