摘要
目的探讨补肾通络的康泉方对实验性大鼠前列腺组织碱性成纤维细胞生长因子(basic fibro-blast growth factor,bFGF)表达的影响。方法采用大鼠去势后注射丙酸睾丸酮致前列腺增生法造模,同时灌胃中药康泉方30天后,取大鼠前列腺组织,采用免疫组化和逆转录聚合酶链式反应(RT-PCR)法分别检测各组前列腺腹叶组织bFGF表达。结果与模型组比较,康泉高、中剂量组bFGF和bFGFmRNA表达明显降低(P<0.05或P<0.01),康泉低剂量组bFGF和bFGFmRNA表达差异无统计学意义(P>0.05)。结论康泉方能够降低实验性良性前列腺增生大鼠前列腺组织bFGF表达。
Objective To investigate the effect of Kangquan Recipe （KQR） on basic fibroblast growth factor （bFGF） in prostatic tissue of rats. Methods Benign prostatic hyperplasia model rat was established by injecting testosterone after castration. After being administered with KQR by gastrogavage for 30 days, the model rats were killed and their abdominal lobe prostate glands were taken for determining the protein and mRNA expressions of bF- GF using immunohistochemical method and RT-polymerase chain reaction （RT-PCR） respectively. Results The expressions of bFGF and bFGF mRNA were significantly lower in the model rats being treated with high or medium dose of KQR than those in the untreated model rats（ P 〈 0.05 or P 〈0.01 ） , but these indexes were insignificantly different between the model rats treated with low dose of KQR and untreated model rats （P 〉 0.05 ）. Conclusion KQR can effectively decrease the expression of bFGF in prostatic tissue of experimental benign prostate hyperplasia model rats.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2008年第8期729-732,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
福建省中医药科研项目(No.wzy0616)
厦门市医学科研项目(No.WSK0526)
厦门市卫生青年创新课题(No.WQK0510)
