摘要
在水温24~26℃条件下,采用半静止式生物毒性实验法,分析了不同浓度的Cu2＋、Zn2＋、Cd2＋对光倒刺鲃（Spinibarbus hollandi）胚胎发育的毒性效应,以及Cu2＋-Cd2＋、Pb2＋-Zn2＋对光倒刺鲃胚胎发育的联合毒性效应。结果表明,在各种不同浓度的重金属离子溶液实验组中胚胎的孵化率均较低,与对照组差异极显著（P〈0.01）;各种重金属在胚胎发育过程中引起各种畸形;除了Cu2＋≥0.10 mg/L、Cu2＋-Cd2＋≥0.50 mg/L、Pb2＋-Zn2＋≥2.00 mg/L实验组会促进胚胎发育速率外,其余各种离子溶液的各实验组均在不同程度上减慢胚胎的发育速率。3种重金属对光倒刺鲃胚胎发育的毒性效应表现为Cu2＋〉Cd2＋〉Zn2＋。Cu2＋、Cd2＋、Zn2＋、Cu2＋-Cd2＋混合、Pb2＋-Zn2＋混合离子对光倒刺鲃胚胎发育的安全浓度分别为0.009 mg/L、0.065 mg/L、0.023 mg/L、0.011 mg/L、0.523 mg/L。Cu2＋与Cd2＋对光倒刺鲃胚胎的联合毒性为拮抗作用。
The present paper deals with the toxic effects of Cu2. , Zn2＋ , Cd2＋ in different ion concentration and the joint toxicity of Cu2＋ -Cd2＋ and Pb2＋ -Zn2＋ on the embryos development of Spinibarbus hoUandi by using the semi- static test method,under the condition of water temperature between 24 - 26~C. The result suggests that the hatching rate of embryos incubated at the heavy metal solutions of different concentration is lower than the control, and the differences is extreme significant （ P 〈 0.01 ）. Malformation of embryos was caused by the heavy metal element during the embryos development stage. The development speed of embryo was advanced in the experimental groups （Cu2＋ /〉 0.10 mg/L,Cu2＋ -Cd2＋ 〉10.50 mg/L,Pb2＋ -Zn2＋ ≥ 2.00 mg/L）, but reduced in the rest of experimental groups of various concentration of metal solutions. The toxic effects of the heavy metals on the embryos development of S. hollandi are Cu2＋ 〉 Cd＋ 〉 Zn2＋ . The safe concentration of Cu2＋ , Cd2＋ , Zn2＋ , Cu2＋ -Cd2. , Pb2＋ -Zn2＋ are 0.009 mg/L,0.065 mg/L,0.023 mg/L,0.01 1 mg/L,0.523 mg/L respectively .The joint toxicity of Cu2＋ and Cd2＋ to the S. hollandi is antagonistic.
出处
《动物学杂志》
CAS
CSCD
北大核心
2008年第4期7-12,共6页
Chinese Journal of Zoology
基金
广东省教育厅资助项目(No.Z02079)
关键词
光倒刺鲃
重金属
胚胎发育
毒性效应
联合毒性
Spinibarbus hollandi
Heavy metals
Embryos development
Toxic effects
Join toxicity
