摘要
目的观察丙泊酚预处理对大鼠离体心脏低温缺血常温再灌注损伤的保护作用。方法采用Langendorff离体心脏灌注模型,60只SD大鼠离体心脏平衡20min后随机分为5组(n=12):空白对照组(Con组)于37℃用Krebs-Henseleit缓冲液(K-H液)持续灌注175min;缺血再灌注组(I/R组)于37℃用K-H液灌注40min,27℃缺血75min,37℃K-H液再灌注60min;丙泊酚预处理1组(P1组)、2组(P2组)和3组(P3组)分别在缺血前给予含50、100和150μmol/L丙泊酚预处理,预处理后同I/R组。观察平衡末(基础值)、缺血前及再灌注末的心率(HR)、左室舒张末压(LVEDP)、左室发展压(LVDP)、左室压力上升和下降速率最大值(±dP/dtmax);在平衡末、再灌注后1、10、20、30及60min分别取冠状动脉流出液,测定其中肌酸激酶(CK)和乳酸脱氢酶(LDH),再灌注末测定心肌组织中超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量;并测定再灌注后各组心肌梗死面积。结果P2组和P3组在再灌注后60min时HR、LVDP、±dP/dtmax、SOD活性均高于I/R组(P<0.05);而LVEDP、心肌梗死面积、MDA含量小于I/R组(P<0.05);P2组和P3组再灌注后各时间点冠状动脉流出液中CK及LDH值均明显低于I/R组相应时间点所测值(P<0.05)。结论100和150μmol/L丙泊酚预处理对大鼠心肌低温缺血再灌注损伤有保护作用。
Objective To investigate the protective effects of propofol preconditioning on myocardium against hypothermia ischemia normothermia reperfusion injury on isolated rat hearts. Methods The Langendorff apparatus was used.Sixty SD rat hearts were divided randomly into 5 groups after 20-minute equilibrium（n=12）： control（Con） group,hearts were continually perfused with K-H buffer for 175 min;ischemia/reperfusion（I/R） group,hearts were perfused with K-H buffer for 40 min,then subjected to global ischemia at 27 ℃for 75 min,and followed reperfusion at 37 ℃for 60 min;propofol preconditioning group 1（P1）,group 2（P2）,and group 3（P3）,hearts were perfused with K-H buffer including 50,100,and 150 μmol/L propofol for 10 min and followed reperfusion like I/R group,respectively.Heart rate（HR）,left ventricular end-diastolic pressure（LVEDP）, left ventricular developed pressure（LVDP） and ±dp/dtmax at the end of equilibration,pre-ischemia and at the end of reperfusion were recorded.The contents of creatine kinase（CK） and lactate dehydrogenase（LDH） in coronary effluent were measured at the end of equilibration and 1,10,20,30,and 60 min during reperfusion.The activity of superoxide dismutase（SOD） and the contents of maleic dialdehyde（MDA） were measured at the end of reperfusion.The area of infarct region was determined at the end of reperfusion. Results HR,LVDP,±dp/dtmax and SOD activity in P2 and P3 group were higher than those in I/R group（P〈0.05）.LVEDP, area of infarct region and MDA content in P2 and P3 group were lower than those in I/R group（P〈0.05）.The contents of CK and LDH in coronary effluent in P2 and P3 group decreased significantly in comparison with the I/R group at each time points（P〈0.05）. Conclusion Propofol preconditioning with 100 μmol/L and 150 μmol/L can mitigate myocardial hypothermia ischemia normothermia reperfusion injury on rat hearts.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2008年第8期992-995,共4页
Journal of Shanghai Jiao tong University:Medical Science
关键词
丙泊酚
预处理
低温
心肌
缺血再灌注损伤
propofol
preconditioning
hypothermia
myocardium
ischemia reperfusion injury
