摘要
目的回顾分析抗核抗体(ANA)与抗细胞核细胞浆抗体谱(ANA谱)在本实验室的应用状况,以及在自身免疫性疾病诊断上的价值。方法ANA用间接免疫荧光法检测,ANA谱用欧蒙印迹法检测。结果312例临床标本中,ANA阳性数为123例,阳性率为39.9%(123/312),核型种类主要为核细颗粒型、均质型和核仁型等,滴度各有不同。312例标本同时做ANA谱检测,阳性数为88例,阳性率为25%(88/312)。阳性抗体出现次数较多的是抗SSA、Ro52、SSB、CENPB(着丝点B蛋白)和Histone(组蛋白)等,基本上与荧光法测定ANA核型的结果相符。只是当ANA为低滴度(起始滴度1:100)时,ANA谱为阴性结果的可能性较高。结论用间接免疫荧光法筛查ANA,再用ANA谱作确认分型,可以更准确地确定ANA靶抗原的类型,ANA谱可检测15种针对细胞核和细胞浆的抗体,同传统的抗ENA抗体六项相比,扩大了检测范围,增强了对ANA的分型能力,具有较高的应用价值。
Objective To review the application of antinudear antibody（ANA） and its profile（ANA profile） and their value in diagnosis of autoimmune diseases.Methods to detect ANA and ANA profde by indirect immunofluorescence based technique（IIF） and Euronline （inanunoblot assay） method respecfively. Results Of all the 312 samples, 123 cases is positive for ANA（123/312, 39.9% ）.There were several types of ANA,the majority of which were homogeneous pattern, free speckled pattern and antinucleolar pattern. The titers of ANA were diverse. Of the 312 samples that were measured ANA and ANA profile simutaneously,88 cases is positive for ANA profile（88/312,25% ）.The most frequently occurred antibody was anti-SSA, Ro52,SSB, CENPB and Histone, and was concordant with the type of ANA. For the samples positive for ANA with low fiters（1：100） ,a negative result would more frequently occurred for ANA profiles. Conclusion to screen ANA with IIF and to confirm the type of antibodies by ANA profile, the types of target antigen of autoantibodies can be accurately determined. To determine autoanfibody against nucleus and cytoplasma with ANA Profile, 15 kinds of autoantibodies can be determined. In contrast to ENA（6 kinds of autoantibodies）, more kinds of antibodies can be detected,which enhanced the ability of ANA typing,so there is more important application value for the usage of ANA profile.
出处
《中国实验诊断学》
2008年第8期1031-1033,共3页
Chinese Journal of Laboratory Diagnosis
