摘要
目的：了解中国新疆吉木萨尔野马繁殖中心的普氏野马（Equus przewalskii）遗传多样性及其遗传背景。方法：采用PCR产物直接测序法，对15匹普氏野马线粒体DNAD—loop高变区进行测序分析。结果：测定15个个体的线粒体DNAD—loop高变区15464—15866片段序列402bp。检测到12种单倍型，包括37个多态位点，占全部序列的9．2％，其中转换位点2，4个、颠换位点20个、转换位点和颠换并存位点8个、缺失位点3个。A％＋T％含量（56．1％）高于G％＋C％含量（43．9％），平均A含量为28．4％，T含量为27．7％，C含量为29％，G含为14．9％。单倍型间平均遗传距离为0．030，单倍型多态性（h）为1±0．00116，核苷酸多态性（7c）为2．90％。15匹普氏野马线粒体DNAD—loop高变区之间平均核苷酸变异率为2．48％。结论：研究表明我国新疆吉木萨尔野马繁殖中心的普氏野马线粒体DNAD—loop区序列存在丰富的多态性。
Objective： To invest/gate the genetic diversity and genetic data of Przewalski＇ s horses from Wild Horse Breeding Center in Jimsar county, Xinjiang Province, China. Method： Sequence polymorphisms in 15 individuals of Przewalski＇ s horses mtDNA D -loop region were deter- mined by PCR production direct sequencing. Result： The mitochondrial D - loop hypervariable segment sequences 15464- 15866 （402bp） in 15 individuals of Przewalski＇s Horse were sequenced. 12 haplotypes were identfied in the samples, with 37 sites showing polymorphism, it accounts for 9.2% in total sequences, which were 24 transitiom, 20 transversion, 8 coexistent sites of transition and transversion and 3 deletions. The contents of A% ＋ T% 56.1% was higher than C% ＋ G% 43.9%. The contents of A, T, G and C were 28.4%,27.7%, 14.9% and 29%, respectively. The haplotypes diversity is 1 ± 0.00116. The nucleotide diversity is 2.90%. The average genetic distance of haplotypes is 0.030. The average nucleotide mutational rate of mtDNA D-loop varied region in 15 individuals of Przewalski＇s horses was 2.48%. Conclu- sion： The results showed that the Przewalski＇ s horses maintain high variation in mitochondrlal D - loop sequences.
出处
《生物技术》
CAS
CSCD
2008年第4期4-7,共4页
Biotechnology
基金
国家自然科学基金项目资助(30660025)
中国科学院昆明动物研究所合作项目资助
