摘要
目的比较鉴定海洛因成瘾者和正常人血浆蛋白质组差异,为研究海洛因成瘾相关血浆蛋白提供线索。方法海洛因成瘾者（n=5）和正常对照者（n=5）血浆蛋白经剔除白蛋白和免疫球蛋白IgG后,以固相pH梯度4-7胶条等电聚焦为第一向,SDS聚丙烯酰胺凝胶电泳为第二向,进行蛋白双向电泳。图像分析软件Image Master Elit 5.0分析蛋白质2维图谱。手工挖取组间相差1.5倍的差异点,串联质谱分析鉴定。结果每张图谱平均检测到350±21个蛋白（亚基）斑点,其中5个蛋白点在2组图谱中差异1.5倍以上,鉴定结果分别为γ纤维蛋白原、人α1B糖蛋白、α1-抗胰蛋白酶原、视黄醇结合蛋白载体蛋白四聚体单体和铜蓝蛋白。结论海洛因成瘾者血浆与正常人血浆对比存在蛋白质组差异。某些差异蛋白可能与海洛因成瘾造成的神经损伤相关。
AIM To identify differentially expressed proteins in the plasma that may be used as biomarkers for heroin addiction through a two-dimensional （2-D） gel electrophoresis/mass spectrometry approach. METHODS Following removal of albumin and IgG, the plasma from 5 heroin abusers and 5 normal controls was separated by 2-D gel electrophoresis using immobilized pH gradients 4 -7 drystrip and PAGE. Gel images were analyzed using ImageMaster Elit 5.0. Differential proteins were selected and analyzed through tandem mass spectrometry. RESULTS Average number for samples was 350± 21 protein spots. In them there were 5 spots that differed by more than 1.5 fold between the two groups obtained through image analysis. Through tandem mass spectrometry above spots were identified as fibrinogen γ （increased by 5 fold）, human α1-B-glycoprotein （decreased to 1/1.8 of control group）, uncleaved α1-antitrypsin （increased by 2.5 fold）, chain of transthyretin （decreased to 1/2 of control group ） and ceruloplasmin （increased by 6. 6 fold ）. CONCLUSION There are differences between heroin abusers and normal controls in the blood plasma proteome. Some proteins may have a role in the damage to central nervous system through heroin abuse. Such proteins may provide novel biomarkers for diagnosis and therapeutic targeting, as well as clues for understanding the mechanism of heroin abuse.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2008年第4期241-249,共9页
Chinese Journal of Pharmacology and Toxicology
基金
Natural Science Foundation of Guangdong Province(06301434)~~
关键词
海洛因依赖
光谱分析
质量
血浆
蛋白质组
电泳
凝胶
双向
heroin dependence
spectrum analysis, mass
plasma
proteome
electrophoresis, gel, two-dimensional
