摘要
目的:探讨甲状腺乳头状癌(PTC)患者BRAFT1799A突变与PDGF、VEGF表达及肿瘤生长方式的关系。方法:对116名甲状腺乳头状癌(PTC)患者的基因进行了分析,并随机选取BRAFT1799A阳性和阴性病例各30名。BRAFT1799A突变采用聚合酶链反应-聚丙烯酰氨凝胶电泳法分析,产物经测序证实。PDGF、VEGF表达采用免疫组织化学方法检测。结果:116名PTC患者中BRAFT1799A阳性者33名,阳性率为28.4%。BRAFT1799A阳性者肿瘤腺体外浸润和肿瘤Ⅲ级以上分级比例高于阴性患者(P<0.05)。BRAFT1799A阳性患者PDGF-B表达水平明显高于阴性患者(P<0.01),而两者PDGF-A和VEGF表达水平无明显差异(P>0.05)。在调整性别、年龄后Logistic回归分析显示BRAFT1799A与肿瘤腺体外浸润和肿瘤Ⅲ级以上分级及PDGF-B表达呈明显正相关。结论:BRAFT1799A影响肿瘤的生长方式,使PTC肿瘤腺体外浸润生长发生率高,进而影响PTC患者临床预后。
Objective：BRAF T1799A mutation may affect the growth style of thyroid cancer and the prognosis of patient. Platelet -derived growth factor （PDGF） and vascular endothelial growth factor （VEGF） are closely correlated with tumor metastasis. This study mainly investigate the relation of BRAF T1799A mutation of papillary thyroid carcinoma （PTC） with expression of PDGF, VEGF and tumor growth style. Methods： The study analyzed the gene of 116 PTC patients, and randomly selected 30 BRAF T1799A positive and negative patients respectively. BRAF T1799A mutation was analyzed with polymerase chain reaction -SSCP method, and the production was confirmed by gene sequencing. The expression of PDGF and VEGF was analyzed by immunohistochemistry. Results： There were 33 BRAF T1799A positive patients in the 116 patients. The positive rate was 28.4 %. The BRAF T1799A （ ＋ ） PTC had a higher rate of extra - thyroidal invasion and Ill/IV tumor stage when compared to BRAF T1799A （ - ） PTC （P 〈0.05）. Although BRAF T1799A （ ＋ ） PTC tissues had higher expression levels of PDGF -B than BRAF T1799A （ - ） PTC tissues, expression levels of PDGF - A and VEGF in BRAF V600E （ ＋ ） PTC were not significantly different from those of BRAF V600E （ - ） PTC （P 〉0. 05）. After adjustments for age, sex, multivariate logistic regression analyses showed BRAF T1799A was positively correlated to extrathyroidal invasion, Ⅲ/Ⅳ tumor stage and expression level of PDGF - B. Conclusion： BRAF T1799A mutation may affect the growth style of thyroid cancer; BRAF T1799A （ ＋ ） PTC had a higher rate of extra -thyroidal invasion and poor clinical prognosis.
出处
《现代肿瘤医学》
CAS
2008年第9期1469-1472,共4页
Journal of Modern Oncology
