摘要
目的 阐明参芪扶正液对肺腺癌细胞株GLC-82X射线照射后TGF-β1表达的调控，探讨中药防治放射性肺损伤的分子机制。方法 选取人低分化肺腺癌细胞株GLC-82，加入终浓度为1．563mg／ml参芪扶正液后随机分组：①照射剂量关系组：分别予0、20、30和40 Gy剂量的X射线照射，于照射后6h提取细胞总RNA。②时间关系组：接受20 Gy的X射线照射，分别在照射后12、24和48h提取细胞总RNA。2组均采用RT-PCR法检测TGF-β1 mRNA表达。结果 剂量关系组：GLC-82细胞接受X射线照射后TGF—β1 mRNA表达量明显增高，在20Gy照射后达到高峰，为基础未照射水平细胞的5倍。参芪扶正干预组与单纯照射组相比，其TGF—β1 mRNA相对表达量均有不同程度下调，在吸收剂量达20 Gy时差异有统计学意义（P〈0．05）。时间关系组：GLC-82细胞经20 Gy X射线照射后在不同时间点参芪扶正干预组与单纯照射组相比，其TGF—β1 mRNA相对表达量均有不同程度下调，受照后48h差异有统计学意义（P〈0．01）。结论 TGF—β1在放射性肺损伤发生、发展过程中起着重要作用，而参芪扶正液能明显降低该过程中TGF—β1表达水平。
Objective To investigate the ability of Shenqi Fuzheng Injection on release of TGF—β1, mRNA in human lung adenocarcinoma GLC- 82 cells by ionizing radiation in vitro, and find the regulation mechanism to reduce the radiation-induced lung injury by traditional Chinese drug. Methods GLC-82 cells were cultured in media with serum containing 1.563 mg/ml Shenqi Fuzheng Injection, then TGF—β1 mRNA after exposure to different irradiation doses （0, 20,30,40 Gy） and time intervals（ 12,24,48 h ） were investigated. The expression of TGF—β1 mRNA detected semi-quantitatively by RT-PCR. Results For the different irradiation doses group, TGF—β1 expression were increased after irradiation, which was significantly elevated at 20 Gy compared with unirradiated cells. The GLC-82 cells, treated by Shenqi Fuzheng Injection, revealed a minor radiation mediated TGF—β1 response on mRNA level, which was significantly different at 20 Gy compared with the irradiated group （P 〈 0.05）. For the different time intervals group, following irradiation exposure in 20 Gy, the TGF—β1 mRNA levels in cells, which were treated by Shenqi Fuzheng Injection, were down-regulated and significantly decrease at 48 h （ P 〈 0.01 ）. Conclusions The transforming growth factor beta 1 -TGF—β1 is a key mediator for the pathogenesis of radiation-induced lung injury. Shenqi Fuzheng Injection down-regulates the radiationinduced TGF—β1 release, and it might reduce the lung toxicity after thoracic irradiation.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2008年第4期358-360,共3页
Chinese Journal of Radiological Medicine and Protection
基金
中国临床肿瘤学科学基金资助项目（Y20050040）
湖北省科技厅科技公关项目（2005AA401C25）
