摘要
目的:探讨抗CXCR4单克隆抗体12G5对SGC7901细胞与基质的粘附性与侵袭性的影响。方法:应用MTT法、Transwell Chamber体外侵袭实验技术检测12G5(5μg/ml、10μg/ml、20μg/ml)对胃癌细胞株SGC7901粘附、侵袭和迁移能力的影响。结果:(1)10μg/ml的12G5即可使胃癌细胞粘附Matrigel的能力明显受抑,与对照组相比(P<0.01)。随着时间的延长及单抗浓度的加大,实验组的粘附能力低于对照组,差异有统计学意义(P<0.01)。(2)SGC7901细胞对照组和实验组侵袭迁移实验穿膜细胞数分别为:121.7±5.51,54.3±9.07;140.3±6.03,62.0±11.53。实验组的侵袭迁移能力明显低于对照组,差异有统计学意义(P<0.01)。结论:12G5能在一定程度上抑制SGC7901细胞的粘附性及侵袭性。
Objective：To investigate the effects of anti-CXCR4 monoclonal antibody 12G5 on adhesion and invasion of SGC7901 cells.Methods ： The effects of 12G5 （ 5μ g/ml, 10μg/ml and 20μg/ml, respectively ）on the adhesive,invasive and migrative abilities of SGC7901 calls were explored by MTT and Transwell chamber. Results： （1）12G5 showed a time/dose-dependent inhibition effect on SGC7901 cells adhering Matrigel,and the adhesion of gastric cancer cells was inhibited on the concentration of 10~g/ml of 12G5, and compared with untreated group,adhesion of SGC7901 cells was signficantly lower（P〈0.01 ）;（2 ）The invaded cell numbers in invasive and metastatic experiments of SGC7901 cell lines untreated or treated with 12G5 were 121.7 ± 5.51,54.3 ± 9.07;and 140.3 ± 6.03,62.0 ±11.53 respectively,the invasive and metastatic abilities of treated group were lower than those of untreated group significantl（P〈0.01 ）. Conclusion：12G5 may inhibit adhesion and invasion of SGC7901 cells to certain extent.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第7期817-820,共4页
Journal of Chongqing Medical University
