摘要
目的建立同时测定血清中摹巴比妥（PB）、苯妥荚（PT）、卡马西平（CBZ）和氯硝西泮浓度的HPLC.方法分析柱为Agilent C18柱（4．6mm×250mm,5μm），流动相为水-甲醇-乙腈（45：45：10），流速为1．0mL·min^-1，检测波长为254nm，柱温30℃、阿普唑仑为内标物。结果PB，PT，CBZ和氯硝西泮的线性范围分别为2．5～80，1．25～40，0.75～24，0.02～0.64μg·mL^-1，最低检测浓度分别为0．2,0．2,0．1，0．005μg·mL^-1，方法回收率分别为99．1％，100.8％，100.1％，98.1％，日内、日间RSD均〈3．5％，绝对回收率分别为91．7％，90．2％，89．9％和89．3％，RSD均〈2％。结论该方法快速、灵敏、买用，适用于治疗药物监测。
OBJECTIVE To establish an internal standard HPLC method for the simultaneous determination of phenobarbital （PB） ,phenytoin（PT） ,carbamazepine （CBZ） and clonazepam in human serum. METHODS Agilent C18 column（4.6 mm × 250 mm,5μm） was used. The mobile phase was water-methanol-acetonitrile （45：45： 10）. The flow rate was 1.0 mL · min^-1 and the detection wavelength was 254 nm. The column temperature was 30 ℃. Alprazolam was selected as the internal standard. RESULTS The linear relation range of PB, PT, CBZ and clonazepam were 2. 5 - 80, 1.25 - 40,0.75 - 24 and 0.02 - 0.64 μg ·mL^-1 , respectively. The limit concentration of detection of PB, PT, CBZ and clonazepam were 0. 2,0. 2,9.1,0. 005 μg · mL^-1, respectively.The average recovery of PB ,PT,CBZ and clonazepam were 99.1% , 100.8% , 100.1% ,98.1% , respectively. The RSD for the inter- and intra-day of four drugs were less than 3.5%. Absolute recovery were 91.7% ,90.2% ,89.9% and 89.3% ,respectively. The RSD were less than 2%. CONCLUSION The method is rapid, sensitive, accurate and practival. It is suitable for therapeutic drug monitoring of PB, PT, CBZ and elonazepam.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2008年第4期336-339,共4页
Chinese Journal of Modern Applied Pharmacy
