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重组Endocan转染MDCK细胞对其表达骨桥蛋白的影响 被引量：2

The transfection of recombinant Endocan to MDCK cells and influence on OPN
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摘要 目的构建Endocan真核表达载体pcDNA3.1-En-docan,转染犬肾小管上皮细胞MDCK,观察其在MDCK中的表达并初步分析其对骨桥蛋白(OPN)表达的影响。方法应用PCR从pET28-Endocan中扩增出Endocan cDNA全长序列,酶切后导入pcDNA 3.1/myc-his A多克隆位点,构建真核表达载体pcDNA3.1-Endocan;瞬时转染MDCK细胞,Western blot检测Endocan和OPN表达。结果经双酶切鉴定及测序分析表明Endocan已经克隆到pcDNA 3.1/myc-his A中。Western blot结果表明转染成功,Endocan表达量增加,且OPN随其而增加。结论Endocan可促进MDCK表达OPN。 Objective Eukaryotic expressing plasmid pcDNA3.1-Endocan was constructed and transfected into MDCK, to observe the expression of Endocan and analyse its influence on OPN. Methods Endocan full length cDNA was amplified using PCR from pet28-Endocan, subcloned into pcDNA3.1/myc-his A, transiently transfected into MDCK cells. Western blot was used to detect the expression of Endocan and OPN. Results The construction of pcDNA3.1-Endocan had been successfully established by double enzyme digesting and sequencing. The trasfected cells expressed more Endocan and OPN. Conclusion MDCK cells express more OPN by promoting Endocan.
作者 陈孝宇 胡超杰 左莉 刘超 张素梅 胡若磊 朱华庆 周青 桂淑玉 汪渊
机构地区 安徽医科大学分子生物学实验室、生物化学教研室和安徽省／省部共建教育部重要遗传病基因资源利用重点实验室 安徽医科大学第一附属医院呼吸内科
出处 《安徽医科大学学报》 CAS 北大核心 2008年第4期381-384,共4页 Acta Universitatis Medicinalis Anhui
基金 安徽省自然科学基金项目(编号:050430705,01043716)
关键词 转染 基因表达 肾肿瘤/遗传学 transfection gene expression kidney neoplasms/genetics
分类号 R343.9 [医药卫生—基础医学] R394.2 [医药卫生—医学遗传学]
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参考文献13

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共引文献19

同被引文献21

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安徽医科大学学报
2008年 第4期

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